Ingrid H. G. ?stensen wrote:
> Hi
>
> I am not sure this is the right place to post this message but maybe
someone have some tips/experience to share.
>
> We have an ongoing discussion regarding the number of replicates
needed when we are using Illumina arrays. It is not easy saying one
right number, but the main problem is this: If one can take advantage
of the 30 something beads on the array can one use only one replica
per sample? Have anyone done anything like this?
Hi, Ingrid.
The replicate beads allow an estimate of the technical variation
_within
an array_ for the _same oligo probe_. They do not allow estimation of
biologic variation or array-to-array variation. If you do not need
estimates of the latter two types of variation, which is not a likely
circumstance, then you will need to do biologic replicates. The
array-to-array variation on illumina, we have found, is quite low, but
it is still probably quite worthwhile to balance an experiment with
respect to the arrays whenever possible to reduce the possibility of a
technical issue producing what looks to be biological effect.
Sean
