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Question: Creating a new instance of oligoSnpSet
0
10.2 years ago by
Steven McKinney310 wrote:
Hello All, I am trying to get some Illumina HumanCNV370-Quad data into VanillaICE to do some copy number analysis. In attempting to create an object of class "oligoSnpSet" I can not seem to specify an annotation that works. e.g. as specified in a vignette > cclss <- + new("oligoSnpSet", copyNumber = logR, calls = gt, + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), + featureData = cclfd, annotation = "Illumina550k") Loading required package: Illumina550k Error in db(object) : Illumina550k package not available In addition: Warning message: In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, : there is no package called 'Illumina550k' Error in dbGetQuery(db(object), sql) : error in evaluating the argument 'conn' in selecting a method for function 'dbGetQuery' or even if I specify some annotation that does exist > cclss <- + new("oligoSnpSet", copyNumber = logR, calls = gt, + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), + featureData = cclfd, annotation = "hgu133plus2cdf") Loading required package: hgu133plus2cdf Error in db(object) : trying to get slot "getdb" from an object of a basic class ("environment") with no slots Error in dbGetQuery(db(object), sql) : error in evaluating the argument 'conn' in selecting a method for function 'dbGetQuery' Is there a way to work around this annotation bit of building an eSet object? I can't figure out from documentation, reading source code, or experimenting, as to what will work for this annotation argument. I'm a bit hooped as there does not yet appear to be annotation for the Illumina HumanCNV370-Quad, but I have annotation information from other files from Illumina etc. Can I put some dummy object as an argument for annotation and patch it up with my known info? Any ideas? Steven McKinney Statistician Molecular Oncology and Breast Cancer Program British Columbia Cancer Research Centre email: smckinney +at+ bccrc +dot+ ca tel: 604-675-8000 x7561 BCCRC Molecular Oncology 675 West 10th Ave, Floor 4 Vancouver B.C. V5Z 1L3 Canada
ADD COMMENTlink
modified 10.2 years ago • written 10.2 years ago by Steven McKinney310
Answer: Creating a new instance of oligoSnpSet
0
10.2 years ago by
Steven McKinney310 wrote:
Hi all, Thanks to Robert Scharpf for a quick and detailed off-line response. For anyone else that may encounter this issue: my problem was that my featureData object's 'data' slot data frame did not have names "chromosome" and "position" . I originally defined my featureData object as > cclfd <- + new("AnnotatedDataFrame", + data = data.frame(position = pData(featureData(ccld)[, "MapInfo"]), + chromosome = pData(featureData(ccld)[, "CHR"]), + stringsAsFactors = FALSE), + varMetadata = data.frame(labelDescription = c("position", "chromosome"))) extracting directly from my ccld object (a SnpSetIllumina object from beadarraySNP command read.SnpSetIllumina() ccld <- read.SnpSetIllumina(samplesheet = "ccl_CNV370SampleSheet_8samples.csv", reportfile = "ccl_FinalReport_2.txt") ) This yielded an AnnotatedDataFrame object with slot 'data' containing a data frame whose names were not those I had put in the data.frame() code above (namely "position" and "chromosome"). > str(cclfd) Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots ..@ varMetadata :'data.frame': 2 obs. of 1 variable: .. ..$labelDescription: chr [1:2] "position" "chromosome" ..@ data :'data.frame': 373397 obs. of 2 variables: .. ..$ MapInfo: num [1:373397] 1.64e+08 1.66e+08 1.66e+08 1.66e+08 1.67e+08 ... .. ..$CHR : Factor w/ 25 levels "1","10","11",..: 18 18 18 18 18 18 18 18 18 18 ... .. .. ..- attr(*, "names")= chr [1:373397] "cnvi0000001" "cnvi0000002" "cnvi0000003" "cnvi0000004" ... ..@ dimLabels : chr [1:2] "rowNames" "columnNames" ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots .. .. ..@ .Data:List of 1 .. .. .. ..$ : int [1:3] 1 1 0 So that's my R lesson for today - names specified in a data.frame() call don't necessarily stick! Explicitly forcing column names and mode "character" for the chromosome column solves the problem ccld.position <- pData(featureData(ccld)[, "MapInfo"]) names(ccld.position) <- "position" ccld.chromosome <- pData(featureData(ccld)[, "CHR"]) names(ccld.chromosome) <- "chromosome" ccld.chromosome$chromosome <- as.character(ccld.chromosome$chromosome) cclfd <- new("AnnotatedDataFrame", data = data.frame(position = ccld.position, chromosome = ccld.chromosome, stringsAsFactors = FALSE), varMetadata = data.frame(labelDescription = c("position", "chromosome"))) and I can create the oligoSnpSet object successfully. > cclss <- + new("oligoSnpSet", copyNumber = logR, calls = gt, + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), + featureData = cclfd, annotation = "HumanCNV370-Quad") > str(cclss) Formal class 'oligoSnpSet' [package "oligoClasses"] with 6 slots So it was the absence of columns named "chromosome" and "position" in the 'data' slot of the featureData object that caused internal code to attempt to acquire chromosome positional information from an annotation source. With the featureData at data data frame having the correct column labels "chromosome" and "position", the annotation argument is not processed further (it is just added to the SnpSet object's 'annotation' slot). Thanks again to Robert Scharpf. Best Steve McKinney -----Original Message----- From: bioconductor-bounces@stat.math.ethz.ch on behalf of Steven McKinney Sent: Tue 11/25/2008 9:56 PM To: Bioconductor at stat.math.ethz.ch Subject: [BioC] Creating a new instance of oligoSnpSet Hello All, I am trying to get some Illumina HumanCNV370-Quad data into VanillaICE to do some copy number analysis. In attempting to create an object of class "oligoSnpSet" I can not seem to specify an annotation that works. e.g. as specified in a vignette > cclss <- + new("oligoSnpSet", copyNumber = logR, calls = gt, + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), + featureData = cclfd, annotation = "Illumina550k") Loading required package: Illumina550k Error in db(object) : Illumina550k package not available In addition: Warning message: In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, : there is no package called 'Illumina550k' Error in dbGetQuery(db(object), sql) : error in evaluating the argument 'conn' in selecting a method for function 'dbGetQuery' or even if I specify some annotation that does exist > cclss <- + new("oligoSnpSet", copyNumber = logR, calls = gt, + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), + featureData = cclfd, annotation = "hgu133plus2cdf") Loading required package: hgu133plus2cdf Error in db(object) : trying to get slot "getdb" from an object of a basic class ("environment") with no slots Error in dbGetQuery(db(object), sql) : error in evaluating the argument 'conn' in selecting a method for function 'dbGetQuery' Is there a way to work around this annotation bit of building an eSet object? I can't figure out from documentation, reading source code, or experimenting, as to what will work for this annotation argument. I'm a bit hooped as there does not yet appear to be annotation for the Illumina HumanCNV370-Quad, but I have annotation information from other files from Illumina etc. Can I put some dummy object as an argument for annotation and patch it up with my known info? Any ideas? Steven McKinney Statistician Molecular Oncology and Breast Cancer Program British Columbia Cancer Research Centre email: smckinney +at+ bccrc +dot+ ca tel: 604-675-8000 x7561 BCCRC Molecular Oncology 675 West 10th Ave, Floor 4 Vancouver B.C. V5Z 1L3 Canada _______________________________________________ Bioconductor mailing list Bioconductor at stat.math.ethz.ch https://stat.ethz.ch/mailman/listinfo/bioconductor Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor
ADD COMMENTlink written 10.2 years ago by Steven McKinney310
Hi Steven -- Steven McKinney wrote: > Hi all, > > Thanks to Robert Scharpf for a quick and detailed > off-line response. For anyone else that may encounter > this issue: my problem was that my featureData object's > 'data' slot data frame did not have names "chromosome" > and "position" . > > I originally defined my featureData object as > >> cclfd <- > + new("AnnotatedDataFrame", > + data = data.frame(position = pData(featureData(ccld)[, "MapInfo"]), > + chromosome = pData(featureData(ccld)[, "CHR"]), > + stringsAsFactors = FALSE), > + varMetadata = data.frame(labelDescription = c("position", "chromosome"))) > > extracting directly from my ccld object (a SnpSetIllumina object > from beadarraySNP command read.SnpSetIllumina() > ccld <- read.SnpSetIllumina(samplesheet = "ccl_CNV370SampleSheet_8samples.csv", > reportfile = "ccl_FinalReport_2.txt") > ) > > > This yielded an AnnotatedDataFrame object with slot 'data' > containing a data frame whose names were not those I had > put in the data.frame() code above (namely "position" > and "chromosome"). > >> str(cclfd) > Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots > ..@ varMetadata :'data.frame': 2 obs. of 1 variable: > .. ..$labelDescription: chr [1:2] "position" "chromosome" > ..@ data :'data.frame': 373397 obs. of 2 variables: > .. ..$ MapInfo: num [1:373397] 1.64e+08 1.66e+08 1.66e+08 1.66e+08 1.67e+08 ... > .. ..$CHR : Factor w/ 25 levels "1","10","11",..: 18 18 18 18 18 18 18 18 18 18 ... > .. .. ..- attr(*, "names")= chr [1:373397] "cnvi0000001" "cnvi0000002" "cnvi0000003" "cnvi0000004" ... > ..@ dimLabels : chr [1:2] "rowNames" "columnNames" > ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots > .. .. ..@ .Data:List of 1 > .. .. .. ..$ : int [1:3] 1 1 0 > > So that's my R lesson for today - names specified in a > data.frame() call don't necessarily stick! Hmm, I'm not sure that's the right lesson -- you don't have to be that suspicious of data.frame. It might be AnnotatedDataFrame or oligoSnpSet, though. I wonder what your sessionInfo() is? Also what does str(featureData(ccld)) say? An unusual thing is the 'names' attribute of cclfd. Any chance of creating a reproducible example (i.e., without access to your files, maybe by referencing help pages [using the 'example()' function] or making a version with just a few features and using dput)? A couple of short-cuts / tips. fData(obj) gives you direct access to pData(featureData(obj)). 'extract-then-subset' fData(obj))[,"cols"] -- will usually be more efficient that subset then extract; there's also a subtle difference that might be causing problems here (as you do it, you end up with a 1-column data frame for 'chromosome', whereas extract-then-subset results in a vector). '[[' pulls out a single column with featureData(obj)[["cols"]] (also [[<- can be useful for defining a single column and creating a labelDescription; obj[["cols"]] gives direct access to pData(obj)[["cols"]]). Martin > Explicitly forcing column names and > mode "character" for the chromosome column > solves the problem > > ccld.position <- pData(featureData(ccld)[, "MapInfo"]) > names(ccld.position) <- "position" > ccld.chromosome <- pData(featureData(ccld)[, "CHR"]) > names(ccld.chromosome) <- "chromosome" > ccld.chromosome$chromosome <- as.character(ccld.chromosome$chromosome) > > cclfd <- > new("AnnotatedDataFrame", > data = data.frame(position = ccld.position, > chromosome = ccld.chromosome, > stringsAsFactors = FALSE), > varMetadata = data.frame(labelDescription = c("position", "chromosome"))) > > and I can create the oligoSnpSet object successfully. > >> cclss <- > + new("oligoSnpSet", copyNumber = logR, calls = gt, > + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), > + featureData = cclfd, annotation = "HumanCNV370-Quad") >> str(cclss) > Formal class 'oligoSnpSet' [package "oligoClasses"] with 6 slots > > > So it was the absence of columns named "chromosome" and "position" > in the 'data' slot of the featureData object that caused internal > code to attempt to acquire chromosome positional information from > an annotation source. > > With the featureData at data data frame having the correct column > labels "chromosome" and "position", the annotation argument > is not processed further (it is just added to the SnpSet > object's 'annotation' slot). > > Thanks again to Robert Scharpf. > > Best > > Steve McKinney > > > > > -----Original Message----- > From: bioconductor-bounces at stat.math.ethz.ch on behalf of Steven McKinney > Sent: Tue 11/25/2008 9:56 PM > To: Bioconductor at stat.math.ethz.ch > Subject: [BioC] Creating a new instance of oligoSnpSet > > Hello All, > > I am trying to get some Illumina HumanCNV370-Quad > data into VanillaICE to do some copy number analysis. > > In attempting to create an object of class "oligoSnpSet" > I can not seem to specify an annotation that works. > > e.g. as specified in a vignette > >> cclss <- > + new("oligoSnpSet", copyNumber = logR, calls = gt, > + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), > + featureData = cclfd, annotation = "Illumina550k") > Loading required package: Illumina550k > Error in db(object) : Illumina550k package not available > In addition: Warning message: > In library(package, lib.loc = lib.loc, character.only = TRUE, logical.return = TRUE, : > there is no package called 'Illumina550k' > Error in dbGetQuery(db(object), sql) : > error in evaluating the argument 'conn' in selecting a method for function 'dbGetQuery' > > or even if I specify some annotation that does exist > >> cclss <- > + new("oligoSnpSet", copyNumber = logR, calls = gt, > + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), > + featureData = cclfd, annotation = "hgu133plus2cdf") > Loading required package: hgu133plus2cdf > Error in db(object) : > trying to get slot "getdb" from an object of a basic class ("environment") with no slots > Error in dbGetQuery(db(object), sql) : > error in evaluating the argument 'conn' in selecting a method for function 'dbGetQuery' > > > Is there a way to work around this annotation bit of building > an eSet object? > > I can't figure out from documentation, reading source code, or > experimenting, as to what will work for this annotation argument. > > I'm a bit hooped as there does not yet appear to be annotation > for the Illumina HumanCNV370-Quad, but I have annotation > information from other files from Illumina etc. > > Can I put some dummy object as an argument for annotation > and patch it up with my known info? > > Any ideas? > > > Steven McKinney > > Statistician > Molecular Oncology and Breast Cancer Program > British Columbia Cancer Research Centre > > email: smckinney +at+ bccrc +dot+ ca > > tel: 604-675-8000 x7561 > > BCCRC > Molecular Oncology > 675 West 10th Ave, Floor 4 > Vancouver B.C. > V5Z 1L3 > Canada > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Martin Morgan Computational Biology / Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109 Location: Arnold Building M2 B169 Phone: (206) 667-2793
ADD REPLYlink written 10.2 years ago by Martin Morgan ♦♦ 22k
Hi Martin, Thanks for your feedback. I've put requested info inline below. I'll work on a reproduce with manageable data. Best Steve McKinney > -----Original Message----- > From: Martin Morgan [mailto:mtmorgan at fhcrc.org] > Sent: Wednesday, November 26, 2008 1:23 PM > To: Steven McKinney > Cc: Bioconductor at stat.math.ethz.ch > Subject: Re: [BioC] Creating a new instance of oligoSnpSet > > Hi Steven -- > > Steven McKinney wrote: > > Hi all, > > > > Thanks to Robert Scharpf for a quick and detailed > > off-line response. For anyone else that may encounter > > this issue: my problem was that my featureData object's > > 'data' slot data frame did not have names "chromosome" > > and "position" . > > > > I originally defined my featureData object as > > > >> cclfd <- > > + new("AnnotatedDataFrame", > > + data = data.frame(position = pData(featureData(ccld)[, > "MapInfo"]), > > + chromosome = pData(featureData(ccld)[, "CHR"]), > > + stringsAsFactors = FALSE), > > + varMetadata = data.frame(labelDescription = c("position", > "chromosome"))) > > > > extracting directly from my ccld object (a SnpSetIllumina object > > from beadarraySNP command read.SnpSetIllumina() > > ccld <- read.SnpSetIllumina(samplesheet = > "ccl_CNV370SampleSheet_8samples.csv", > > reportfile = "ccl_FinalReport_2.txt") > > ) > > > > > > This yielded an AnnotatedDataFrame object with slot 'data' > > containing a data frame whose names were not those I had > > put in the data.frame() code above (namely "position" > > and "chromosome"). > > > >> str(cclfd) > > Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots > > ..@ varMetadata :'data.frame': 2 obs. of 1 variable: > > .. ..$labelDescription: chr [1:2] "position" "chromosome" > > ..@ data :'data.frame': 373397 obs. of 2 variables: > > .. ..$ MapInfo: num [1:373397] 1.64e+08 1.66e+08 1.66e+08 1.66e+08 > 1.67e+08 ... > > .. ..$CHR : Factor w/ 25 levels "1","10","11",..: 18 18 18 18 18 > 18 18 18 18 18 ... > > .. .. ..- attr(*, "names")= chr [1:373397] "cnvi0000001" "cnvi0000002" > "cnvi0000003" "cnvi0000004" ... > > ..@ dimLabels : chr [1:2] "rowNames" "columnNames" > > ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with > 1 slots > > .. .. ..@ .Data:List of 1 > > .. .. .. ..$ : int [1:3] 1 1 0 > > > > So that's my R lesson for today - names specified in a > > data.frame() call don't necessarily stick! > > Hmm, I'm not sure that's the right lesson -- you don't have to be that > suspicious of data.frame. > foo <- data.frame(fooCol1 = 1:3) > bar <- data.frame(barCol1 = 11:13) > baz <- data.frame(a = foo, b = bar) > baz fooCol1 barCol1 1 1 11 2 2 12 3 3 13 > I thought the column names 'a' and 'b' specified in the baz data frame construction would be used, but this is not the case. Not suspicious, just surprised. This behaviour is not indicated nor contra-indicated in the data.frame() documentation, so it's just one of those lessons learned by trial. > > It might be AnnotatedDataFrame or oligoSnpSet, though. I wonder what > your sessionInfo() is? Also what does str(featureData(ccld)) say? An > unusual thing is the 'names' attribute of cclfd. Any chance of creating > a reproducible example (i.e., without access to your files, maybe by > referencing help pages [using the 'example()' function] or making a > version with just a few features and using dput)? > str(featureData(ccld)) Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots ..@ varMetadata :'data.frame': 4 obs. of 1 variable: .. ..$labelDescription: chr [1:4] "CHR" "MapInfo" "GTS" "OPA" ..@ data :'data.frame': 373397 obs. of 4 variables: .. ..$ CHR : Factor w/ 25 levels "1","10","11",..: 18 18 18 18 18 18 18 18 18 18 ... .. .. ..- attr(*, "names")= chr [1:373397] "cnvi0000001" "cnvi0000002" "cnvi0000003" "cnvi0000004" ... .. ..$MapInfo: num [1:373397] 1.64e+08 1.66e+08 1.66e+08 1.66e+08 1.67e+08 ... .. ..$ GTS : num [1:373397] 0 0 0 0 0 0 0 0 0 0 ... .. ..$OPA : Factor w/ 1 level "HumanCNV370-Quadv3_C": 1 1 1 1 1 1 1 1 1 1 ... ..@ dimLabels : chr [1:2] "featureNames" "featureColumns" ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with 1 slots .. .. ..@ .Data:List of 1 .. .. .. ..$ : int [1:3] 1 1 0 > sessionInfo() R version 2.8.0 Patched (2008-11-06 r46845) powerpc-apple-darwin9.5.0 locale: C attached base packages: [1] tools stats graphics grDevices utils datasets methods [8] base other attached packages: [1] RSQLite_0.7-1 DBI_0.2-4 VanillaICE_1.4.0 SNPchip_1.6.0 [5] oligoClasses_1.4.0 Biobase_2.2.1 loaded via a namespace (and not attached): [1] AnnotationDbi_1.4.1 SparseM_0.78 [3] affy_1.20.0 affyio_1.10.1 [5] annotate_1.20.1 beadarraySNP_1.8.0 [7] grid_2.8.0 illuminaHumanv3.db_1.1.2 [9] illuminaHumanv3BeadID.db_1.1.2 limma_2.16.3 [11] lumi_1.8.3 lumiHumanAll.db_1.4.0 [13] lumiHumanIDMapping_1.0.1 mgcv_1.4-1 [15] preprocessCore_1.4.0 quantsmooth_1.8.0 [17] xtable_1.5-4 > > > A couple of short-cuts / tips. fData(obj) gives you direct access to > pData(featureData(obj)). 'extract-then-subset' fData(obj))[,"cols"] -- > will usually be more efficient that subset then extract; there's also a > subtle difference that might be causing problems here (as you do it, you > end up with a 1-column data frame for 'chromosome', whereas > extract-then-subset results in a vector). '[[' pulls out a single column > with featureData(obj)[["cols"]] (also [[<- can be useful for defining a > single column and creating a labelDescription; obj[["cols"]] gives > direct access to pData(obj)[["cols"]]). I will work these ideas into my script. Thanks much for the pointers. > > Martin > > > Explicitly forcing column names and > > mode "character" for the chromosome column > > solves the problem > > > > ccld.position <- pData(featureData(ccld)[, "MapInfo"]) > > names(ccld.position) <- "position" > > ccld.chromosome <- pData(featureData(ccld)[, "CHR"]) > > names(ccld.chromosome) <- "chromosome" > > ccld.chromosome$chromosome <- as.character(ccld.chromosome$chromosome) > > > > cclfd <- > > new("AnnotatedDataFrame", > > data = data.frame(position = ccld.position, > > chromosome = ccld.chromosome, > > stringsAsFactors = FALSE), > > varMetadata = data.frame(labelDescription = c("position", > "chromosome"))) > > > > and I can create the oligoSnpSet object successfully. > > > >> cclss <- > > + new("oligoSnpSet", copyNumber = logR, calls = gt, > > + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), > > + featureData = cclfd, annotation = "HumanCNV370-Quad") > >> str(cclss) > > Formal class 'oligoSnpSet' [package "oligoClasses"] with 6 slots > > > > > > So it was the absence of columns named "chromosome" and "position" > > in the 'data' slot of the featureData object that caused internal > > code to attempt to acquire chromosome positional information from > > an annotation source. > > > > With the featureData at data data frame having the correct column > > labels "chromosome" and "position", the annotation argument > > is not processed further (it is just added to the SnpSet > > object's 'annotation' slot). > > > > Thanks again to Robert Scharpf. > > > > Best > > > > Steve McKinney > > > > > > > > > > -----Original Message----- > > From: bioconductor-bounces at stat.math.ethz.ch on behalf of Steven > McKinney > > Sent: Tue 11/25/2008 9:56 PM > > To: Bioconductor at stat.math.ethz.ch > > Subject: [BioC] Creating a new instance of oligoSnpSet > > > > Hello All, > > > > I am trying to get some Illumina HumanCNV370-Quad > > data into VanillaICE to do some copy number analysis. > > > > In attempting to create an object of class "oligoSnpSet" > > I can not seem to specify an annotation that works. > > > > e.g. as specified in a vignette > > > >> cclss <- > > + new("oligoSnpSet", copyNumber = logR, calls = gt, > > + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), > > + featureData = cclfd, annotation = "Illumina550k") > > Loading required package: Illumina550k > > Error in db(object) : Illumina550k package not available > > In addition: Warning message: > > In library(package, lib.loc = lib.loc, character.only = TRUE, > logical.return = TRUE, : > > there is no package called 'Illumina550k' > > Error in dbGetQuery(db(object), sql) : > > error in evaluating the argument 'conn' in selecting a method for > function 'dbGetQuery' > > > > or even if I specify some annotation that does exist > > > >> cclss <- > > + new("oligoSnpSet", copyNumber = logR, calls = gt, > > + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), > > + featureData = cclfd, annotation = "hgu133plus2cdf") > > Loading required package: hgu133plus2cdf > > Error in db(object) : > > trying to get slot "getdb" from an object of a basic class > ("environment") with no slots > > Error in dbGetQuery(db(object), sql) : > > error in evaluating the argument 'conn' in selecting a method for > function 'dbGetQuery' > > > > > > Is there a way to work around this annotation bit of building > > an eSet object? > > > > I can't figure out from documentation, reading source code, or > > experimenting, as to what will work for this annotation argument. > > > > I'm a bit hooped as there does not yet appear to be annotation > > for the Illumina HumanCNV370-Quad, but I have annotation > > information from other files from Illumina etc. > > > > Can I put some dummy object as an argument for annotation > > and patch it up with my known info? > > > > Any ideas? > > > > > > Steven McKinney > > > > Statistician > > Molecular Oncology and Breast Cancer Program > > British Columbia Cancer Research Centre > > > > email: smckinney +at+ bccrc +dot+ ca > > > > tel: 604-675-8000 x7561 > > > > BCCRC > > Molecular Oncology > > 675 West 10th Ave, Floor 4 > > Vancouver B.C. > > V5Z 1L3 > > Canada > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: > http://news.gmane.org/gmane.science.biology.informatics.conductor > > > -- > Martin Morgan > Computational Biology / Fred Hutchinson Cancer Research Center > 1100 Fairview Ave. N. > PO Box 19024 Seattle, WA 98109 > > Location: Arnold Building M2 B169 > Phone: (206) 667-2793
ADD REPLYlink written 10.2 years ago by Steven McKinney310
Thanks Steve for persisting... cutting to the chase Steven McKinney wrote: > Hi Martin, > > Thanks for your feedback. > > I've put requested info inline below. > I'll work on a reproduce with manageable > data. > >>> So that's my R lesson for today - names specified in a >>> data.frame() call don't necessarily stick! >> Hmm, I'm not sure that's the right lesson -- you don't have to be that >> suspicious of data.frame. > > > foo <- data.frame(fooCol1 = 1:3) > > bar <- data.frame(barCol1 = 11:13) > > baz <- data.frame(a = foo, b = bar) > > baz > fooCol1 barCol1 > 1 1 11 > 2 2 12 > 3 3 13 Sorry, my mistake, I guess this is the essence of your problem. I thought I'd convinced myself that this was not the case, but I got it wrong -- columns really are being named based on foo and bar, rather than argument names. Martin > > I thought the column names 'a' and 'b' specified in the > baz data frame construction would be used, but > this is not the case. Not suspicious, just surprised. > This behaviour is not indicated nor contra-indicated > in the data.frame() documentation, so it's just one > of those lessons learned by trial. > >> It might be AnnotatedDataFrame or oligoSnpSet, though. I wonder what >> your sessionInfo() is? Also what does str(featureData(ccld)) say? An >> unusual thing is the 'names' attribute of cclfd. Any chance of > creating >> a reproducible example (i.e., without access to your files, maybe by >> referencing help pages [using the 'example()' function] or making a >> version with just a few features and using dput)? > >> str(featureData(ccld)) > Formal class 'AnnotatedDataFrame' [package "Biobase"] with 4 slots > ..@ varMetadata :'data.frame': 4 obs. of 1 variable: > .. ..$labelDescription: chr [1:4] "CHR" "MapInfo" "GTS" "OPA" > ..@ data :'data.frame': 373397 obs. of 4 variables: > .. ..$ CHR : Factor w/ 25 levels "1","10","11",..: 18 18 18 18 18 > 18 18 18 18 18 ... > .. .. ..- attr(*, "names")= chr [1:373397] "cnvi0000001" "cnvi0000002" > "cnvi0000003" "cnvi0000004" ... > .. ..$MapInfo: num [1:373397] 1.64e+08 1.66e+08 1.66e+08 1.66e+08 > 1.67e+08 ... > .. ..$ GTS : num [1:373397] 0 0 0 0 0 0 0 0 0 0 ... > .. ..$OPA : Factor w/ 1 level "HumanCNV370-Quadv3_C": 1 1 1 1 1 1 > 1 1 1 1 ... > ..@ dimLabels : chr [1:2] "featureNames" "featureColumns" > ..@ .__classVersion__:Formal class 'Versions' [package "Biobase"] with > 1 slots > .. .. ..@ .Data:List of 1 > .. .. .. ..$ : int [1:3] 1 1 0 > >> sessionInfo() > R version 2.8.0 Patched (2008-11-06 r46845) > powerpc-apple-darwin9.5.0 > > locale: > C > > attached base packages: > [1] tools stats graphics grDevices utils datasets methods > > [8] base > > other attached packages: > [1] RSQLite_0.7-1 DBI_0.2-4 VanillaICE_1.4.0 > SNPchip_1.6.0 > [5] oligoClasses_1.4.0 Biobase_2.2.1 > > loaded via a namespace (and not attached): > [1] AnnotationDbi_1.4.1 SparseM_0.78 > [3] affy_1.20.0 affyio_1.10.1 > [5] annotate_1.20.1 beadarraySNP_1.8.0 > [7] grid_2.8.0 illuminaHumanv3.db_1.1.2 > [9] illuminaHumanv3BeadID.db_1.1.2 limma_2.16.3 > [11] lumi_1.8.3 lumiHumanAll.db_1.4.0 > [13] lumiHumanIDMapping_1.0.1 mgcv_1.4-1 > [15] preprocessCore_1.4.0 quantsmooth_1.8.0 > [17] xtable_1.5-4 > >> A couple of short-cuts / tips. fData(obj) gives you direct access to >> pData(featureData(obj)). 'extract-then-subset' fData(obj))[,"cols"] -- >> will usually be more efficient that subset then extract; there's also > a >> subtle difference that might be causing problems here (as you do it, > you >> end up with a 1-column data frame for 'chromosome', whereas >> extract-then-subset results in a vector). '[[' pulls out a single > column >> with featureData(obj)[["cols"]] (also [[<- can be useful for defining > a >> single column and creating a labelDescription; obj[["cols"]] gives >> direct access to pData(obj)[["cols"]]). > > I will work these ideas into my script. Thanks much for the pointers. > >> Martin >> >>> Explicitly forcing column names and >>> mode "character" for the chromosome column >>> solves the problem >>> >>> ccld.position <- pData(featureData(ccld)[, "MapInfo"]) >>> names(ccld.position) <- "position" >>> ccld.chromosome <- pData(featureData(ccld)[, "CHR"]) >>> names(ccld.chromosome) <- "chromosome" >>> ccld.chromosome$chromosome <- > as.character(ccld.chromosome$chromosome) >>> cclfd <- >>> new("AnnotatedDataFrame", >>> data = data.frame(position = ccld.position, >>> chromosome = ccld.chromosome, >>> stringsAsFactors = FALSE), >>> varMetadata = data.frame(labelDescription = c("position", >> "chromosome"))) >>> and I can create the oligoSnpSet object successfully. >>> >>>> cclss <- >>> + new("oligoSnpSet", copyNumber = logR, calls = gt, >>> + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), >>> + featureData = cclfd, annotation = "HumanCNV370-Quad") >>>> str(cclss) >>> Formal class 'oligoSnpSet' [package "oligoClasses"] with 6 slots >>> >>> >>> So it was the absence of columns named "chromosome" and "position" >>> in the 'data' slot of the featureData object that caused internal >>> code to attempt to acquire chromosome positional information from >>> an annotation source. >>> >>> With the featureData at data data frame having the correct column >>> labels "chromosome" and "position", the annotation argument >>> is not processed further (it is just added to the SnpSet >>> object's 'annotation' slot). >>> >>> Thanks again to Robert Scharpf. >>> >>> Best >>> >>> Steve McKinney >>> >>> >>> >>> >>> -----Original Message----- >>> From: bioconductor-bounces at stat.math.ethz.ch on behalf of Steven >> McKinney >>> Sent: Tue 11/25/2008 9:56 PM >>> To: Bioconductor at stat.math.ethz.ch >>> Subject: [BioC] Creating a new instance of oligoSnpSet >>> >>> Hello All, >>> >>> I am trying to get some Illumina HumanCNV370-Quad >>> data into VanillaICE to do some copy number analysis. >>> >>> In attempting to create an object of class "oligoSnpSet" >>> I can not seem to specify an annotation that works. >>> >>> e.g. as specified in a vignette >>> >>>> cclss <- >>> + new("oligoSnpSet", copyNumber = logR, calls = gt, >>> + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), >>> + featureData = cclfd, annotation = "Illumina550k") >>> Loading required package: Illumina550k >>> Error in db(object) : Illumina550k package not available >>> In addition: Warning message: >>> In library(package, lib.loc = lib.loc, character.only = TRUE, >> logical.return = TRUE, : >>> there is no package called 'Illumina550k' >>> Error in dbGetQuery(db(object), sql) : >>> error in evaluating the argument 'conn' in selecting a method for >> function 'dbGetQuery' >>> or even if I specify some annotation that does exist >>> >>>> cclss <- >>> + new("oligoSnpSet", copyNumber = logR, calls = gt, >>> + phenoData = annotatedDataFrameFrom(logR, byrow = FALSE), >>> + featureData = cclfd, annotation = "hgu133plus2cdf") >>> Loading required package: hgu133plus2cdf >>> Error in db(object) : >>> trying to get slot "getdb" from an object of a basic class >> ("environment") with no slots >>> Error in dbGetQuery(db(object), sql) : >>> error in evaluating the argument 'conn' in selecting a method for >> function 'dbGetQuery' >>> >>> Is there a way to work around this annotation bit of building >>> an eSet object? >>> >>> I can't figure out from documentation, reading source code, or >>> experimenting, as to what will work for this annotation argument. >>> >>> I'm a bit hooped as there does not yet appear to be annotation >>> for the Illumina HumanCNV370-Quad, but I have annotation >>> information from other files from Illumina etc. >>> >>> Can I put some dummy object as an argument for annotation >>> and patch it up with my known info? >>> >>> Any ideas? >>> >>> >>> Steven McKinney >>> >>> Statistician >>> Molecular Oncology and Breast Cancer Program >>> British Columbia Cancer Research Centre >>> >>> email: smckinney +at+ bccrc +dot+ ca >>> >>> tel: 604-675-8000 x7561 >>> >>> BCCRC >>> Molecular Oncology >>> 675 West 10th Ave, Floor 4 >>> Vancouver B.C. >>> V5Z 1L3 >>> Canada >>> >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at stat.math.ethz.ch >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >>> _______________________________________________ >>> Bioconductor mailing list >>> Bioconductor at stat.math.ethz.ch >>> https://stat.ethz.ch/mailman/listinfo/bioconductor >>> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> >> >> -- >> Martin Morgan >> Computational Biology / Fred Hutchinson Cancer Research Center >> 1100 Fairview Ave. N. >> PO Box 19024 Seattle, WA 98109 >> >> Location: Arnold Building M2 B169 >> Phone: (206) 667-2793 -- Martin Morgan Computational Biology / Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109 Location: Arnold Building M2 B169 Phone: (206) 667-2793
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