Error "Specified environment does not contain MoGene-1_0-st-v1"
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Peng Yu ▴ 940
@peng-yu-3586
Last seen 9.7 years ago
Hi, I have some .cel files and MoGene-1_0-st-v1,r3.cdf in the current directory. The .cel files are generated from the Mouse Gene 1.0 ST Array. I run the following command. But it generate an error message. Can somebody let me know what the problem is? Regards, Peng > setwd(".") > library(makecdfenv) > data<-ReadAffy() > eset<-rma(data) Error in getCdfInfo(object) : ? Could not obtain CDF environment, problems encountered: Specified environment does not contain MoGene-1_0-st-v1 Library - package mogene10stv1cdf not installed Bioconductor - mogene10stv1cdf not available
cdf cdf • 1.4k views
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@vincent-j-carey-jr-4
Last seen 6 weeks ago
United States
you'll need to use the oligo package to work with that data. source("http://bioconductor.org/biocLite.R") biocLite("oligo", "pd.mogene.1.0.st.v1") then oligo's read.celfiles() and rma() can be used. you may also need biocLite("mogene10stprobeset.db") to help interpret results On Wed, Jul 22, 2009 at 2:37 PM, Peng Yu <pengyu.ut at="" gmail.com=""> wrote: > > Hi, > > I have some .cel files and MoGene-1_0-st-v1,r3.cdf in the current > directory. The .cel files are generated from the Mouse Gene 1.0 ST > Array. > > I run the following command. But it generate an error message. Can > somebody let me know what the problem is? > > Regards, > Peng > > > setwd(".") > > library(makecdfenv) > > data<-ReadAffy() > > eset<-rma(data) > Error in getCdfInfo(object) : > ? Could not obtain CDF environment, problems encountered: > Specified environment does not contain MoGene-1_0-st-v1 > Library - package mogene10stv1cdf not installed > Bioconductor - mogene10stv1cdf not available > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- Vincent Carey, PhD Biostatistics, Channing Lab 617 525 2265
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Hi, I am still new to bioc. Could you please point me the most relevant document? Shall I start with the following document? http://www.bioconductor.org/packages/release/bioc/vignettes/oligo/inst /doc/oligoNimblegenExpression.pdf Regards, Peng On Wed, Jul 22, 2009 at 1:43 PM, Vincent Carey <stvjc at="" channing.harvard.edu=""> wrote: > > you'll need to use the oligo package to work with that data. > > ? ?source("http://bioconductor.org/biocLite.R") > ? ?biocLite("oligo", "pd.mogene.1.0.st.v1") > > then oligo's read.celfiles() and rma() can be used. ?you may also need > > biocLite("mogene10stprobeset.db") > > to help interpret results > > On Wed, Jul 22, 2009 at 2:37 PM, Peng Yu <pengyu.ut at="" gmail.com=""> wrote: > > > > Hi, > > > > I have some .cel files and MoGene-1_0-st-v1,r3.cdf in the current > > directory. The .cel files are generated from the Mouse Gene 1.0 ST > > Array. > > > > I run the following command. But it generate an error message. Can > > somebody let me know what the problem is? > > > > Regards, > > Peng > > > > > setwd(".") > > > library(makecdfenv) > > > data<-ReadAffy() > > > eset<-rma(data) > > Error in getCdfInfo(object) : > > ? Could not obtain CDF environment, problems encountered: > > Specified environment does not contain MoGene-1_0-st-v1 > > Library - package mogene10stv1cdf not installed > > Bioconductor - mogene10stv1cdf not available > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > > > -- > Vincent Carey, PhD > Biostatistics, Channing Lab > 617 525 2265
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@james-w-macdonald-5106
Last seen 1 day ago
United States
Hi Peng, Peng Yu wrote: > Hi, > > I have some .cel files and MoGene-1_0-st-v1,r3.cdf in the current > directory. The .cel files are generated from the Mouse Gene 1.0 ST > Array. > > I run the following command. But it generate an error message. Can > somebody let me know what the problem is? > > Regards, > Peng > >> setwd(".") >> library(makecdfenv) >> data<-ReadAffy() >> eset<-rma(data) > Error in getCdfInfo(object) : > Could not obtain CDF environment, problems encountered: > Specified environment does not contain MoGene-1_0-st-v1 > Library - package mogene10stv1cdf not installed > Bioconductor - mogene10stv1cdf not available Although the affy package can read these data in, it won't be able to process the data without extra (unnecessary) work from you, as the affy package wasn't designed to analyze these chips. Please see here: http://www.bioconductor.org/docs/workflows/oligoarrays/ For a listing of packages that are designed for this chip. Best, Jim > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor -- James W. MacDonald, M.S. Biostatistician Douglas Lab University of Michigan Department of Human Genetics 5912 Buhl 1241 E. Catherine St. Ann Arbor MI 48109-5618 734-615-7826
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