Entering edit mode
Ivanek, Robert
▴
100
@ivanek-robert-3765
Last seen 10.3 years ago
Dear John, BioC-community,
I tried to run the runBioHMM function on my data (Nimblegen HD 2.1
Arrays), however I am always getting the error: "Error: segfault from
C
stack overflow".
What is the reason for that error? Are the data too big? I tried to
split the array according the chromosomes but it did not help. Only
after splitting the data into small pieces the function run
successfully.
I preprocessed the data according the snapCGH manual
(snapCGHguide.pdf),
below is the code that produced the error, object class and
sessionInfo.
Best Regards
Robert
--
Robert Ivanek
Postdoctoral Fellow Schuebeler Group
Friedrich Miescher Institute
Maulbeerstrasse 66
4058 Basel / Switzerland
Office phone: +41 61 697 6100
######################################################################
##########
R> SegInfo.BioHMM <- runBioHMM(MA.cgh2)
sample is 1 Chromosomes: 1 2 Error: segfault from C stack
overflow
######################################################################
##########
R> class(MA.cgh2)
[1] "SegList"
attr(,"package")
[1] "snapCGH"
######################################################################
##########
R> dim(MA.cgh2$M)
[1] 1967673 10
######################################################################
##########
R> MA.cgh2
An object of class "SegList"
$state
NULL
$rpred
NULL
$prob
NULL
$M.predicted
NULL
$dispersion
NULL
$variance
NULL
$M.observed
a b c
d e f g
[1,] -0.12942320 0.02683309 -0.10259011
0.22068827 0.1938552 0.09126507 0.08812914
[2,] -0.13219905 0.34312167 0.21092262
0.08040469 -0.2627170 -0.05179436 0.28135520
[3,] 0.07700826 -0.01331928 0.06368898
0.12494860 0.1382679 0.20195685 -0.05675671
[4,] 0.14806237 -0.25316790 -0.10510554
0.01550725 0.2686752 0.16356962 -0.38026647
[5,] 0.22431210 -0.39476064 -0.17044853
-0.29215523 0.1026054 -0.06784313 -0.34597137
h i j
[1,] 0.06129606 -0.04129405 -0.13255913
[2,] -0.06176647 0.14915615 0.20095051
[3,] -0.04343744 0.02025154 -0.18170531
[4,] -0.12709857 -0.23220410 -0.39577372
[5,] 0.04878927 -0.12165927 -0.05381614
1967668 more rows ...
$genes
Block GENE_EXPR_OPTION PROBE_ID POSITION Row Column Status
ID Chr Position
2 1 BLOCK1 CHR01FS003044249 3044249 266 3118 BLOCK1
CHR01FS003044249 1 3.044249
4 1 BLOCK1 CHR01FS003090598 3090598 454 3038 BLOCK1
CHR01FS003090598 1 3.090598
7 1 BLOCK1 CHR01FS003090918 3090918 109 3793 BLOCK1
CHR01FS003090918 1 3.090918
9 1 BLOCK1 CHR01FS003091098 3091098 14 3674 BLOCK1
CHR01FS003091098 1 3.091098
10 1 BLOCK1 CHR01FS003091218 3091218 1047 3673 BLOCK1
CHR01FS003091218 1 3.091218
1967668 more rows ...
$M
a b c
d e f g
[1,] -0.12942320 0.02683309 -0.10259011
0.22068827 0.1938552 0.09126507 0.08812914
[2,] -0.13219905 0.34312167 0.21092262
0.08040469 -0.2627170 -0.05179436 0.28135520
[3,] 0.07700826 -0.01331928 0.06368898
0.12494860 0.1382679 0.20195685 -0.05675671
[4,] 0.14806237 -0.25316790 -0.10510554
0.01550725 0.2686752 0.16356962 -0.38026647
[5,] 0.22431210 -0.39476064 -0.17044853
-0.29215523 0.1026054 -0.06784313 -0.34597137
h i j
[1,] 0.06129606 -0.04129405 -0.13255913
[2,] -0.06176647 0.14915615 0.20095051
[3,] -0.04343744 0.02025154 -0.18170531
[4,] -0.12709857 -0.23220410 -0.39577372
[5,] 0.04878927 -0.12165927 -0.05381614
1967668 more rows ...
$design
[1] 1 1 1 1 1 1 1 1 1 1
######################################################################
##########
R> sessionInfo()
R version 2.10.1 (2009-12-14)
x86_64-unknown-linux-gnu
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8 LC_MONETARY=C
[6] LC_MESSAGES=en_US.UTF-8 LC_PAPER=en_US.UTF-8 LC_NAME=C
LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] splines stats graphics grDevices utils datasets
methods
base
other attached packages:
[1] RMySQL_0.7-4 DBI_0.2-5 snapCGH_1.16.0
aCGH_1.22.0 multtest_2.2.0 survival_2.35-8
cluster_1.12.1
[8] GLAD_2.6.0 DNAcopy_1.20.0 tilingArray_1.24.0
pixmap_0.4-10 Biobase_2.6.1 limma_3.2.2
RColorBrewer_1.0-2
loaded via a namespace (and not attached):
[1] affy_1.24.2 affyio_1.14.0 annotate_1.24.1
AnnotationDbi_1.8.1 genefilter_1.28.2 grid_2.10.1
lattice_0.18-3
[8] MASS_7.3-5 preprocessCore_1.8.0 RSQLite_0.8-4
strucchange_1.4-0 tools_2.10.1 vsn_3.14.0
xtable_1.5-6