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Guido Hooiveld
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4.1k
@guido-hooiveld-2020
Last seen 13 hours ago
Wageningen University, Wageningen, the …
Dear list,
I am trying to build an annotation package for the Mouse Gene 1.1 ST
Array Plate (GeneTitan peg array) using pdInfoBuilder. As input i use
the PGF, CLF, etc files downloaded from Affymetrix. However, the
following lines of code do not work; specifically I am puzzled by the
error message that a file is in the wrong "format". Can someone please
explain what this error means (i have never heard of
'ScalarCharacter'),
and how to solve it?
Thanks,
Guido
http://www.affymetrix.com/browse/products.jsp?productId=131555&navMode
=3
4000&navAction=jump&aId=productsNav#1_3
> library("pdInfoBuilder")
Loading required package: Biobase
Welcome to Bioconductor
Vignettes contain introductory material. To view, type
'openVignette()'. To cite Bioconductor, see
'citation("Biobase")' and for packages 'citation(pkgname)'.
Loading required package: RSQLite
Loading required package: DBI
Loading required package: affxparser
Loading required package: oligo
Loading required package: oligoClasses
Loading required package: preprocessCore
Welcome to oligo version 1.10.0
>
> pgfFile = "MoGene-1_1-st-v1.r4.pgf";
> clfFile = "MoGene-1_1-st-v1.r4.clf";
> probeFile = "MoGene-1_1-st-v1.mm8.probe.tab";
> transFile = "MoGene-1_1-st-v1.na30.1.mm9.transcript.csv";
> pkg <- new("AffyGenePDInfoPkgSeed",
+ version="0.0.1",
+ author="Guido Hooiveld", email=guido.hooiveld@xxx,
+ biocViews="AnnotationData",
+ genomebuild="mm8",
+ pgfFile=pgfFile, clfFile=clfFile,
+ probeFile=probeFile, transFile=transFile);
Error in validObject(.Object) :
invalid class "AffyGenePDInfoPkgSeed" object: invalid object for
slot
"transFile"
in class "AffyGenePDInfoPkgSeed": got class "character",
should be or extend class "ScalarCharacter"
> sessionInfo()
R version 2.10.1 (2009-12-14)
x86_64-unknown-linux-gnu
locale:
[1] LC_CTYPE=en_US.UTF-8 LC_NUMERIC=C
[3] LC_TIME=en_US.UTF-8 LC_COLLATE=en_US.UTF-8
[5] LC_MONETARY=C LC_MESSAGES=en_US.UTF-8
[7] LC_PAPER=en_US.UTF-8 LC_NAME=C
[9] LC_ADDRESS=C LC_TELEPHONE=C
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C
attached base packages:
[1] stats graphics grDevices utils datasets methods base
other attached packages:
[1] pdInfoBuilder_1.10.1 oligo_1.10.0 preprocessCore_1.8.0
[4] oligoClasses_1.8.0 affxparser_1.18.0 RSQLite_0.8-0
[7] DBI_0.2-5 Biobase_2.6.1
loaded via a namespace (and not attached):
[1] affyio_1.14.0 Biostrings_2.14.10 IRanges_1.4.9
splines_2.10.1
[5] tools_2.10.1
------------------------------------------------
Guido Hooiveld, PhD
Nutrition, Metabolism & Genomics Group
Division of Human Nutrition
Wageningen University
Biotechnion, Bomenweg 2
NL-6703 HD Wageningen
the Netherlands
tel: (+)31 317 485788
fax: (+)31 317 483342
internet: http://nutrigene.4t.com <http: nutrigene.4t.com=""/>
email: guido.hooiveld@wur.nl
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