crlmm package help for copy number affymetrix snp 6.0
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@emilie-sohier-4120
Last seen 9.6 years ago
Hello, i am a French bioinformatics student. I want to use the crlmm package to make the copy number of Genome-Wide Human SNP Array 6.0 Affymetrix but when i execute functions : gensnp=genotype(file.path("SNP6",filessnp),cdfName="genomewidesnp6",co pynumber=TRUE) cnSet=crlmmCopynumber(gensnp) cn=copyNumber(cnSet) But i have the result cn ..... SNP_A-1989634 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA SNP_A-1989647 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA SNP_A-1989659 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA SNP_A-4266322 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA SNP_A-1989663 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA SNP_A-1989664 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA ..... And for the genotype i haven't any problems. Mi session Info: R version 2.11.1 (2010-05-31) x86_64-unknown-linux-gnu locale: [1] C attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] crlmm_1.6.2 oligoClasses_1.10.0 Biobase_2.8.0 loaded via a namespace (and not attached): [1] AnnotationDbi_1.10.1 Biostrings_2.16.4 DBI_0.2-5 [4] IRanges_1.6.6 RSQLite_0.9-1 affyio_1.16.0 [7] annotate_1.26.0 bit_1.1-4 ellipse_0.3-5 [10] ff_2.1-2 genefilter_1.30.0 mvtnorm_0.9-9 [13] preprocessCore_1.10.0 splines_2.11.1 survival_2.35-8 [16] tools_2.11.1 xtable_1.5-6 Warning messages: 1: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible 2: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible Thanks for your help. Emilie
SNP crlmm SNP crlmm • 1.4k views
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@benilton-carvalho-1375
Last seen 4.1 years ago
Brazil/Campinas/UNICAMP
Dear Emilie, please check the documentation of crlmmCopynumber and note the MIN.SAMPLES argument. How many samples do you currently have? b On 18 June 2010 13:20, emilie sohier <emilie.sohier15 at="" orange.fr=""> wrote: > Hello, i am a French bioinformatics student. > > I want to use the crlmm package to make the copy number of Genome- Wide Human SNP Array 6.0 Affymetrix > but when i execute functions : > > gensnp=genotype(file.path("SNP6",filessnp),cdfName="genomewidesnp6", copynumber=TRUE) > cnSet=crlmmCopynumber(gensnp) > cn=copyNumber(cnSet) > > > But i have the result > cn > ..... > SNP_A-1989634 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > SNP_A-1989647 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > SNP_A-1989659 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > SNP_A-4266322 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > SNP_A-1989663 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > SNP_A-1989664 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > ..... > > > > And for the genotype i haven't any problems. > > Mi session Info: > > R version 2.11.1 (2010-05-31) > x86_64-unknown-linux-gnu > > locale: > [1] C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] crlmm_1.6.2 oligoClasses_1.10.0 Biobase_2.8.0 > > loaded via a namespace (and not attached): > [1] AnnotationDbi_1.10.1 Biostrings_2.16.4 DBI_0.2-5 > [4] IRanges_1.6.6 RSQLite_0.9-1 affyio_1.16.0 > [7] annotate_1.26.0 bit_1.1-4 ellipse_0.3-5 > [10] ff_2.1-2 genefilter_1.30.0 mvtnorm_0.9-9 > [13] preprocessCore_1.10.0 splines_2.11.1 survival_2.35-8 > [16] tools_2.11.1 xtable_1.5-6 > Warning messages: > 1: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible > 2: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible > > > Thanks for your help. > Emilie > > _______________________________________________ > Bioconductor mailing list > Bioconductor at stat.math.ethz.ch > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >
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I'have 95 samples. Emilie > Message du 18/06/10 14:37 > De : "Benilton Carvalho" > A : "emilie sohier" > Copie ? : "bioconductor" > Objet : Re: [BioC] crlmm package help for copy number affymetrix snp 6.0 > > > Dear Emilie, > > please check the documentation of crlmmCopynumber and note the > MIN.SAMPLES argument. > > How many samples do you currently have? > > b > > On 18 June 2010 13:20, emilie sohier wrote: > > Hello, i am a French bioinformatics student. > > > > I want to use the crlmm package to make the copy number of Genome- Wide Human SNP Array 6.0 Affymetrix > > but when i execute functions : > > > > gensnp=genotype(file.path("SNP6",filessnp),cdfName="genomewidesnp6 ",copynumber=TRUE) > > cnSet=crlmmCopynumber(gensnp) > > cn=copyNumber(cnSet) > > > > > > But i have the result > > cn > > ..... > > SNP_A-1989634 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > > SNP_A-1989647 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > > SNP_A-1989659 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > > SNP_A-4266322 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > > SNP_A-1989663 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > > SNP_A-1989664 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > > ..... > > > > > > > > And for the genotype i haven't any problems. > > > > Mi session Info: > > > > R version 2.11.1 (2010-05-31) > > x86_64-unknown-linux-gnu > > > > locale: > > [1] C > > > > attached base packages: > > [1] stats graphics grDevices utils datasets methods base > > > > other attached packages: > > [1] crlmm_1.6.2 oligoClasses_1.10.0 Biobase_2.8.0 > > > > loaded via a namespace (and not attached): > > [1] AnnotationDbi_1.10.1 Biostrings_2.16.4 DBI_0.2-5 > > [4] IRanges_1.6.6 RSQLite_0.9-1 affyio_1.16.0 > > [7] annotate_1.26.0 bit_1.1-4 ellipse_0.3-5 > > [10] ff_2.1-2 genefilter_1.30.0 mvtnorm_0.9-9 > > [13] preprocessCore_1.10.0 splines_2.11.1 survival_2.35-8 > > [16] tools_2.11.1 xtable_1.5-6 > > Warning messages: > > 1: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible > > 2: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible > > > > > > Thanks for your help. > > Emilie > > > > _______________________________________________ > > Bioconductor mailing list > > Bioconductor at stat.math.ethz.ch > > https://stat.ethz.ch/mailman/listinfo/bioconductor > > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > > > >
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What's the result of: summary(gensnp$SNR) ? On 18 June 2010 13:44, emilie sohier <emilie.sohier15 at="" orange.fr=""> wrote: > > I'have 95 samples. > > Emilie > >> Message du 18/06/10 14:37 >> De : "Benilton Carvalho" >> A : "emilie sohier" >> Copie ? : "bioconductor" >> Objet : Re: [BioC] crlmm package help for copy number affymetrix snp 6.0 >> >> >> Dear Emilie, >> >> please check the documentation of crlmmCopynumber and note the >> MIN.SAMPLES argument. >> >> How many samples do you currently have? >> >> b >> >> On 18 June 2010 13:20, emilie sohier ?wrote: >> > Hello, i am a French bioinformatics student. >> > >> > I want to use the crlmm package to make the copy number of Genome-Wide Human SNP Array 6.0 Affymetrix >> > but when i execute functions : >> > >> > gensnp=genotype(file.path("SNP6",filessnp),cdfName="genomewidesnp 6",copynumber=TRUE) >> > cnSet=crlmmCopynumber(gensnp) >> > cn=copyNumber(cnSet) >> > >> > >> > But i have the result >> > cn >> > ..... >> > SNP_A-1989634 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> > SNP_A-1989647 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> > SNP_A-1989659 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> > SNP_A-4266322 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> > SNP_A-1989663 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> > SNP_A-1989664 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> > ..... >> > >> > >> > >> > And for the genotype i haven't any problems. >> > >> > Mi session Info: >> > >> > R version 2.11.1 (2010-05-31) >> > x86_64-unknown-linux-gnu >> > >> > locale: >> > [1] C >> > >> > attached base packages: >> > [1] stats graphics grDevices utils datasets methods base >> > >> > other attached packages: >> > [1] crlmm_1.6.2 oligoClasses_1.10.0 Biobase_2.8.0 >> > >> > loaded via a namespace (and not attached): >> > [1] AnnotationDbi_1.10.1 Biostrings_2.16.4 DBI_0.2-5 >> > [4] IRanges_1.6.6 RSQLite_0.9-1 affyio_1.16.0 >> > [7] annotate_1.26.0 bit_1.1-4 ellipse_0.3-5 >> > [10] ff_2.1-2 genefilter_1.30.0 mvtnorm_0.9-9 >> > [13] preprocessCore_1.10.0 splines_2.11.1 survival_2.35-8 >> > [16] tools_2.11.1 xtable_1.5-6 >> > Warning messages: >> > 1: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible >> > 2: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible >> > >> > >> > Thanks for your help. >> > Emilie >> > >> > _______________________________________________ >> > Bioconductor mailing list >> > Bioconductor at stat.math.ethz.ch >> > https://stat.ethz.ch/mailman/listinfo/bioconductor >> > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >> > >> >> > > >
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The result is : > summary(gensnp$SNR) Min. 1st Qu. Median Mean 3rd Qu. Max. 2.895 4.784 5.343 5.408 6.052 8.696 > Message du 18/06/10 15:03 > De : "Benilton Carvalho" > A : "emilie sohier" > Copie ? : "bioconductor" > Objet : Re: [BioC] crlmm package help for copy number affymetrix snp 6.0 > > > What's the result of: > > summary(gensnp$SNR) > > ? > > On 18 June 2010 13:44, emilie sohier wrote: > > > > I'have 95 samples. > > > > Emilie > > > >> Message du 18/06/10 14:37 > >> De : "Benilton Carvalho" > >> A : "emilie sohier" > >> Copie ? : "bioconductor" > >> Objet : Re: [BioC] crlmm package help for copy number affymetrix snp 6.0 > >> > >> > >> Dear Emilie, > >> > >> please check the documentation of crlmmCopynumber and note the > >> MIN.SAMPLES argument. > >> > >> How many samples do you currently have? > >> > >> b > >> > >> On 18 June 2010 13:20, emilie sohier ?wrote: > >> > Hello, i am a French bioinformatics student. > >> > > >> > I want to use the crlmm package to make the copy number of Genome-Wide Human SNP Array 6.0 Affymetrix > >> > but when i execute functions : > >> > > >> > gensnp=genotype(file.path("SNP6",filessnp),cdfName="genomewides np6",copynumber=TRUE) > >> > cnSet=crlmmCopynumber(gensnp) > >> > cn=copyNumber(cnSet) > >> > > >> > > >> > But i have the result > >> > cn > >> > ..... > >> > SNP_A-1989634 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > >> > SNP_A-1989647 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > >> > SNP_A-1989659 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > >> > SNP_A-4266322 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > >> > SNP_A-1989663 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > >> > SNP_A-1989664 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA > >> > ..... > >> > > >> > > >> > > >> > And for the genotype i haven't any problems. > >> > > >> > Mi session Info: > >> > > >> > R version 2.11.1 (2010-05-31) > >> > x86_64-unknown-linux-gnu > >> > > >> > locale: > >> > [1] C > >> > > >> > attached base packages: > >> > [1] stats graphics grDevices utils datasets methods base > >> > > >> > other attached packages: > >> > [1] crlmm_1.6.2 oligoClasses_1.10.0 Biobase_2.8.0 > >> > > >> > loaded via a namespace (and not attached): > >> > [1] AnnotationDbi_1.10.1 Biostrings_2.16.4 DBI_0.2-5 > >> > [4] IRanges_1.6.6 RSQLite_0.9-1 affyio_1.16.0 > >> > [7] annotate_1.26.0 bit_1.1-4 ellipse_0.3-5 > >> > [10] ff_2.1-2 genefilter_1.30.0 mvtnorm_0.9-9 > >> > [13] preprocessCore_1.10.0 splines_2.11.1 survival_2.35-8 > >> > [16] tools_2.11.1 xtable_1.5-6 > >> > Warning messages: > >> > 1: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible > >> > 2: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible > >> > > >> > > >> > Thanks for your help. > >> > Emilie > >> > > >> > _______________________________________________ > >> > Bioconductor mailing list > >> > Bioconductor at stat.math.ethz.ch > >> > https://stat.ethz.ch/mailman/listinfo/bioconductor > >> > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > >> > > >> > >> > > > > > > > >
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after off-list communication with Emilie, the proposed solution is for her to appropriately set the 'batch' argument on the genotype() call. b On 18 June 2010 14:08, emilie sohier <emilie.sohier15 at="" orange.fr=""> wrote: > The result is : > >> summary(gensnp$SNR) > Min. 1st Qu. Median Mean 3rd Qu. Max. > 2.895 4.784 5.343 5.408 6.052 8.696 > > > > >> Message du 18/06/10 15:03 >> De : "Benilton Carvalho" >> A : "emilie sohier" >> Copie ? : "bioconductor" >> Objet : Re: [BioC] crlmm package help for copy number affymetrix snp 6.0 >> >> >> What's the result of: >> >> summary(gensnp$SNR) >> >> ? >> >> On 18 June 2010 13:44, emilie sohier ?wrote: >> > >> > I'have 95 samples. >> > >> > Emilie >> > >> >> Message du 18/06/10 14:37 >> >> De : "Benilton Carvalho" >> >> A : "emilie sohier" >> >> Copie ? : "bioconductor" >> >> Objet : Re: [BioC] crlmm package help for copy number affymetrix snp 6.0 >> >> >> >> >> >> Dear Emilie, >> >> >> >> please check the documentation of crlmmCopynumber and note the >> >> MIN.SAMPLES argument. >> >> >> >> How many samples do you currently have? >> >> >> >> b >> >> >> >> On 18 June 2010 13:20, emilie sohier ?wrote: >> >> > Hello, i am a French bioinformatics student. >> >> > >> >> > I want to use the crlmm package to make the copy number of Genome-Wide Human SNP Array 6.0 Affymetrix >> >> > but when i execute functions : >> >> > >> >> > gensnp=genotype(file.path("SNP6",filessnp),cdfName="genomewide snp6",copynumber=TRUE) >> >> > cnSet=crlmmCopynumber(gensnp) >> >> > cn=copyNumber(cnSet) >> >> > >> >> > >> >> > But i have the result >> >> > cn >> >> > ..... >> >> > SNP_A-1989634 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> >> > SNP_A-1989647 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> >> > SNP_A-1989659 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> >> > SNP_A-4266322 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> >> > SNP_A-1989663 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> >> > SNP_A-1989664 NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA NA >> >> > ..... >> >> > >> >> > >> >> > >> >> > And for the genotype i haven't any problems. >> >> > >> >> > Mi session Info: >> >> > >> >> > R version 2.11.1 (2010-05-31) >> >> > x86_64-unknown-linux-gnu >> >> > >> >> > locale: >> >> > [1] C >> >> > >> >> > attached base packages: >> >> > [1] stats graphics grDevices utils datasets methods base >> >> > >> >> > other attached packages: >> >> > [1] crlmm_1.6.2 oligoClasses_1.10.0 Biobase_2.8.0 >> >> > >> >> > loaded via a namespace (and not attached): >> >> > [1] AnnotationDbi_1.10.1 Biostrings_2.16.4 DBI_0.2-5 >> >> > [4] IRanges_1.6.6 RSQLite_0.9-1 affyio_1.16.0 >> >> > [7] annotate_1.26.0 bit_1.1-4 ellipse_0.3-5 >> >> > [10] ff_2.1-2 genefilter_1.30.0 mvtnorm_0.9-9 >> >> > [13] preprocessCore_1.10.0 splines_2.11.1 survival_2.35-8 >> >> > [16] tools_2.11.1 xtable_1.5-6 >> >> > Warning messages: >> >> > 1: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible >> >> > 2: 'DESCRIPTION' file has 'Encoding' field and re-encoding is not possible >> >> > >> >> > >> >> > Thanks for your help. >> >> > Emilie >> >> > >> >> > _______________________________________________ >> >> > Bioconductor mailing list >> >> > Bioconductor at stat.math.ethz.ch >> >> > https://stat.ethz.ch/mailman/listinfo/bioconductor >> >> > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor >> >> > >> >> >> >> >> > >> > >> > >> >> > > >
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