Hi Elspeth, There are three modes regarding how data were stored. C correlated multivariate histogram L List mode U Uncorrelated univariate histograms Currently flowCore only supports 'L' mode, that's why you are getting error. Is it possible to change the mode in which your data were acquired from the machine? Mike Jiang,PhD System Analyst PS Statistics, Vaccine and Infectious Disease Division Fred Hutchinson Cancer Research Center (206)667-1905 On 10/12/2012 03:59 PM, Elspeth Murday wrote: > Hi, > > I apologize if I have contacted you in error, but you are listed as > the person maintaining the flowCore package for R. > > I am having an issue opening any of my FCS files with the read.FCS > utility. I gathered the data using a Partec PAI machine, and have > attached an example file. > > Here is an example of the error I receive: > > > sampdat <- readFCS("A0006980.FCS") > Error in readFCSdata(con, offsets, txt) : > Don't know how to deal with $MODE U > > > If you know of any way that I can circumvent this error to open the > files, I would very much appreciate your input. Thank you! > > Sincerely, > Elspeth Murday > emurday@g.clemson.edu <mailto:emurday@g.clemson.edu> > Clemson University [[alternative HTML version deleted]]