Question: how to annotate Illumina HumanHT-12 v3 chips?
5.1 years ago by
Feng Tian • 110
Feng Tian • 110 wrote:
Hi all list, I want to annotate Illumina HumanHT-12 v3 chips by using the annotation file download from Illumina. The Illumina probes are classified by RefSeq NM Coding transcript, well-established annotation XM Coding transcript, provisional annotation NR Non-coding transcript, well-established annotation XR Non-coding transcript, provisional annotation Supplementary Content UniGene (Build 199) Experimentally confirmed mRNA sequences that align to EST clusters. I have the following questions 1) Should I use all kinds of these probes? Should I only use the RefSeq NM probes? 2) If different kinds of probes (such as RefSeq NM and RefSeq XM) are mapped to the same gene, how to combine them? Thanks. [[alternative HTML version deleted]]
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