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@jayakumar-sivalingam-6161
Last seen 9.6 years ago
Hello, Please let me know about the bioconductor packages to be used for the following things: 1. Quality check of the whole genome raw data 2. Alignment 3. SNP identification 4. Annotation Also, please let me know how I can be able to practice the above things with the example data and the system requirements for the same. Thanks & regards, Jai [[alternative HTML version deleted]]
SNP SNP • 692 views
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@martin-morgan-1513
Last seen 2 hours ago
United States
On 9/25/2013 3:45 PM, jayakumar sivalingam wrote: > Hello, > Please let me know about the bioconductor packages to be used for the > following things: > > > 1. Quality check of the whole genome raw data > 2. Alignment > 3. SNP identification In addition there is VariantTools (Linux only) http://bioconductor.org/packages/release/bioc/html/VariantTools.html > 4. Annotation http://bioconductor.org/help/workflows/variants/ also of interest http://bioconductor.org/packages/release/bioc/html/AnnotationHub.html http://bioconductor.org/help/community/ http://bioconductor.org/help/course-materials/2013/SeattleMay2013/ > > Also, please let me know how I can be able to practice the above things > with the example data and the system requirements for the same. Many packages come with sample data sets; many vignettes and work flows are fully executable, look for the 'R script' on package help pages and package landing pages. Packages usually operate 'across platforms' and many do not have third-party software requirements, although there are exceptions; actual system resources (e.g., memory) used vary by package and problem. Martin > > Thanks & regards, > Jai > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > -- Dr. Martin Morgan, PhD Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109
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Hi, I am trying through the packages that's been suggested. My sincere thankfulness for your guidance. Thanks & regards, Jayakumar S On Thu, Sep 26, 2013 at 12:29 PM, Martin Morgan <mtmorgan@fhcrc.org> wrote: > On 9/25/2013 3:45 PM, jayakumar sivalingam wrote: > >> Hello, >> Please let me know about the bioconductor packages to be used for the >> following things: >> >> >> 1. Quality check of the whole genome raw data >> 2. Alignment >> 3. SNP identification >> > > In addition there is VariantTools (Linux only) > > http://bioconductor.org/**packages/release/bioc/html/**VariantTool s.html<http: bioconductor.org="" packages="" release="" bioc="" html="" varianttools="" .html=""> > > 4. Annotation >> > > http://bioconductor.org/help/**workflows/variants/<http: biocondu="" ctor.org="" help="" workflows="" variants=""/> > > also of interest > > http://bioconductor.org/**packages/release/bioc/html/** > AnnotationHub.html<http: bioconductor.org="" packages="" release="" bioc="" htm="" l="" annotationhub.html=""> > http://bioconductor.org/help/**community/<http: bioconductor.org="" help="" community=""/> > http://bioconductor.org/help/**course- materials/2013/**SeattleMay2013/<http: bioconductor.org="" help="" course-="" materials="" 2013="" seattlemay2013=""/> > > > >> Also, please let me know how I can be able to practice the above things >> with the example data and the system requirements for the same. >> > > Many packages come with sample data sets; many vignettes and work flows > are fully executable, look for the 'R script' on package help pages and > package landing pages. > > Packages usually operate 'across platforms' and many do not have > third-party software requirements, although there are exceptions; actual > system resources (e.g., memory) used vary by package and problem. > > Martin > > > >> Thanks & regards, >> Jai >> >> [[alternative HTML version deleted]] >> >> ______________________________**_________________ >> Bioconductor mailing list >> Bioconductor@r-project.org >> https://stat.ethz.ch/mailman/**listinfo/bioconductor<https: stat.e="" thz.ch="" mailman="" listinfo="" bioconductor=""> >> Search the archives: http://news.gmane.org/gmane.** >> science.biology.informatics.**conductor<http: news.gmane.org="" gmane="" .science.biology.informatics.conductor=""> >> >> > > -- > Dr. Martin Morgan, PhD > Fred Hutchinson Cancer Research Center > 1100 Fairview Ave. N. > PO Box 19024 Seattle, WA 98109 > [[alternative HTML version deleted]]
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Wei Shi ★ 3.6k
@wei-shi-2183
Last seen 10 days ago
Australia/Melbourne/Olivia Newton-John …
Dear jayakumar, The Rsubread package seems to be able to do most of your tasks. Have a look at its users guide for details about the read alignments it performs: library(Rsubread) RsubreadUsersGuide() It has two functions to check the quality of sequencing data: ?qualityScores ?atgcContent It can also detect SNPs: ?callSNPs Its users guide also includes a case study for analyzing RNA-seq data, including read alignment, read summarization and differential expression analysis. Best wishes, Wei On Sep 26, 2013, at 8:45 AM, jayakumar sivalingam wrote: > Hello, > Please let me know about the bioconductor packages to be used for the > following things: > > > 1. Quality check of the whole genome raw data > 2. Alignment > 3. SNP identification > 4. Annotation > > Also, please let me know how I can be able to practice the above things > with the example data and the system requirements for the same. > > Thanks & regards, > Jai > > [[alternative HTML version deleted]] > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor ______________________________________________________________________ The information in this email is confidential and intend...{{dropped:6}}
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