Rbowtie - compilation error
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Hi, I'm trying to install "Rbowtie" package, following the instructions here : http://bioconductor.org/packages/2.12/bioc/html/Rbowtie.html When I run the command, I got this error message : http://pastebin.com/DkEdCV05 I'm currently running on Ubuntu 12.4 LTS and all others packages seems to work well. I updated gcc and g++ to the last version aviable. May you help me to identify the problem ? Thanks. -- output of sessionInfo(): R version 3.1.0 (2014-04-10) Platform: i686-pc-linux-gnu (32-bit) locale: [1] LC_CTYPE=fr_FR.UTF-8 LC_NUMERIC=C [3] LC_TIME=fr_FR.UTF-8 LC_COLLATE=fr_FR.UTF-8 [5] LC_MONETARY=fr_FR.UTF-8 LC_MESSAGES=fr_FR.UTF-8 [7] LC_PAPER=fr_FR.UTF-8 LC_NAME=C [9] LC_ADDRESS=C LC_TELEPHONE=C [11] LC_MEASUREMENT=fr_FR.UTF-8 LC_IDENTIFICATION=C attached base packages: [1] stats graphics grDevices utils datasets methods base other attached packages: [1] BiocInstaller_1.14.2 loaded via a namespace (and not attached): [1] tools_3.1.0 -- Sent via the guest posting facility at bioconductor.org.
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@martin-morgan-1513
Last seen 22 hours ago
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Please remember to include the package maintainer in mail about individual packages. The maintainer is available from the package 'landing page' http://bioconductor.org/packages/release/bioc/html/Rbowtie.html and is cc'd here. There is another Rbowtie issue reported on StackOverflow http://stackoverflow.com/questions/24016722 and https://stat.ethz.ch/pipermail/bioconductor/2014-June/059913.html On 06/04/2014 12:20 AM, Romain [guest] wrote: > Hi, > > I'm trying to install "Rbowtie" package, following the instructions here : http://bioconductor.org/packages/2.12/bioc/html/Rbowtie.html > > When I run the command, I got this error message : > http://pastebin.com/DkEdCV05 > > I'm currently running on Ubuntu 12.4 LTS and all others packages seems to work well. I updated gcc and g++ to the last version aviable. > May you help me to identify the problem ? > > Thanks. > > -- output of sessionInfo(): > > R version 3.1.0 (2014-04-10) > Platform: i686-pc-linux-gnu (32-bit) > > locale: > [1] LC_CTYPE=fr_FR.UTF-8 LC_NUMERIC=C > [3] LC_TIME=fr_FR.UTF-8 LC_COLLATE=fr_FR.UTF-8 > [5] LC_MONETARY=fr_FR.UTF-8 LC_MESSAGES=fr_FR.UTF-8 > [7] LC_PAPER=fr_FR.UTF-8 LC_NAME=C > [9] LC_ADDRESS=C LC_TELEPHONE=C > [11] LC_MEASUREMENT=fr_FR.UTF-8 LC_IDENTIFICATION=C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] BiocInstaller_1.14.2 > > loaded via a namespace (and not attached): > [1] tools_3.1.0 > > > -- > Sent via the guest posting facility at bioconductor.org. > > _______________________________________________ > Bioconductor mailing list > Bioconductor at r-project.org > https://stat.ethz.ch/mailman/listinfo/bioconductor > Search the archives: http://news.gmane.org/gmane.science.biology.informatics.conductor > -- Computational Biology / Fred Hutchinson Cancer Research Center 1100 Fairview Ave. N. PO Box 19024 Seattle, WA 98109 Location: Arnold Building M1 B861 Phone: (206) 667-2793
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Thanks Martin for notifying! Michael On 04.06.2014 13:22, Martin Morgan wrote: > Please remember to include the package maintainer in mail about > individual packages. The maintainer is available from the package > 'landing page' > > http://bioconductor.org/packages/release/bioc/html/Rbowtie.html > > and is cc'd here. > > There is another Rbowtie issue reported on StackOverflow > > http://stackoverflow.com/questions/24016722 > > and > > https://stat.ethz.ch/pipermail/bioconductor/2014-June/059913.html > > On 06/04/2014 12:20 AM, Romain [guest] wrote: >> Hi, >> >> I'm trying to install "Rbowtie" package, following the instructions >> here : http://bioconductor.org/packages/2.12/bioc/html/Rbowtie.html >> >> When I run the command, I got this error message : >> http://pastebin.com/DkEdCV05 >> >> I'm currently running on Ubuntu 12.4 LTS and all others packages seems >> to work well. I updated gcc and g++ to the last version aviable. >> May you help me to identify the problem ? >> >> Thanks. >> >> -- output of sessionInfo(): >> >> R version 3.1.0 (2014-04-10) >> Platform: i686-pc-linux-gnu (32-bit) >> >> locale: >> [1] LC_CTYPE=fr_FR.UTF-8 LC_NUMERIC=C >> [3] LC_TIME=fr_FR.UTF-8 LC_COLLATE=fr_FR.UTF-8 >> [5] LC_MONETARY=fr_FR.UTF-8 LC_MESSAGES=fr_FR.UTF-8 >> [7] LC_PAPER=fr_FR.UTF-8 LC_NAME=C >> [9] LC_ADDRESS=C LC_TELEPHONE=C >> [11] LC_MEASUREMENT=fr_FR.UTF-8 LC_IDENTIFICATION=C >> >> attached base packages: >> [1] stats graphics grDevices utils datasets methods base >> >> other attached packages: >> [1] BiocInstaller_1.14.2 >> >> loaded via a namespace (and not attached): >> [1] tools_3.1.0 >> >> >> -- >> Sent via the guest posting facility at bioconductor.org. >> >> _______________________________________________ >> Bioconductor mailing list >> Bioconductor at r-project.org >> https://stat.ethz.ch/mailman/listinfo/bioconductor >> Search the archives: >> http://news.gmane.org/gmane.science.biology.informatics.conductor >> > >
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@michael-stadler-5887
Last seen 2.3 years ago
Switzerland
Dear Romain, Thank you for the report. I am also using Ubuntu 12.04 LTS, and cannot reproduce your issue, however I am using 64-bit R, while you are using a 32-bit version. Could you test the following things to narrow down the problem: 1. what is the output of: uname -a 2. can you compile bowtie 1.0.1? For this, first download bowtie-1.0.1-src.zip from https://sourceforge.net/projects/bowtie-bio/files/bowtie/1.0.1/ and then change on the shell to the directory containing the downloaded file and run the following commands: unzip bowtie-1.0.1-src.zip cd bowtie-1.0.1/ make Please send me the console output of the 'make'. In my case, I get something like: g++ -O3 -m64 ... -DPOPCNT_CAPABILITY ... and then I find the bowtie binaries: ./bowtie --version which produces: ./bowtie version 1.0.1 64-bit Built on f022c-852634 Don Jun 5 08:47:09 CEST 2014 Compiler: gcc version 4.6.3 (Ubuntu/Linaro 4.6.3-1ubuntu5) Options: -O3 -m64 -Wl,--hash-style=both -DPOPCNT_CAPABILITY Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} 3. if the above does not work, try building again using: cd .. rm -rf bowtie-1.0.1 unzip bowtie-1.0.1-src.zip cd bowtie-1.0.1/ POPCNT_CAPABILITY=0 make and I can test with: ./bowtie version 1.0.1 which gives: 64-bit Built on f022c-852634 Don Jun 5 08:50:34 CEST 2014 Compiler: gcc version 4.6.3 (Ubuntu/Linaro 4.6.3-1ubuntu5) Options: -O3 -m64 -Wl,--hash-style=both Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} Let me know if this is not clear. Best, Michael On 04.06.2014 09:20, Romain [guest] wrote: > Hi, > > I'm trying to install "Rbowtie" package, following the instructions here : http://bioconductor.org/packages/2.12/bioc/html/Rbowtie.html > > When I run the command, I got this error message : > http://pastebin.com/DkEdCV05 > > I'm currently running on Ubuntu 12.4 LTS and all others packages seems to work well. I updated gcc and g++ to the last version aviable. > May you help me to identify the problem ? > > Thanks. > > -- output of sessionInfo(): > > R version 3.1.0 (2014-04-10) > Platform: i686-pc-linux-gnu (32-bit) > > locale: > [1] LC_CTYPE=fr_FR.UTF-8 LC_NUMERIC=C > [3] LC_TIME=fr_FR.UTF-8 LC_COLLATE=fr_FR.UTF-8 > [5] LC_MONETARY=fr_FR.UTF-8 LC_MESSAGES=fr_FR.UTF-8 > [7] LC_PAPER=fr_FR.UTF-8 LC_NAME=C > [9] LC_ADDRESS=C LC_TELEPHONE=C > [11] LC_MEASUREMENT=fr_FR.UTF-8 LC_IDENTIFICATION=C > > attached base packages: > [1] stats graphics grDevices utils datasets methods base > > other attached packages: > [1] BiocInstaller_1.14.2 > > loaded via a namespace (and not attached): > [1] tools_3.1.0 > > > -- > Sent via the guest posting facility at bioconductor.org. > -- -------------------------------------------- Michael Stadler, PhD Head of Computational Biology Friedrich Miescher Institute Basel (Switzerland) Phone : +41 61 697 6492 Fax : +41 61 697 3976 Mail : michael.stadler at fmi.ch
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Hi, here is the output of uname -a : *"Linux retureau-MacBookPro 3.2.0-63-generic-pae #95-Ubuntu SMP Thu May 15 23:26:11 UTC 2014 i686 i686 i386 GNU/Linux**"* I didn't specify it, but i'm running ubuntu in dual bot on a mac book pro. Here is the output of "make" inside the right folder : http://pastebin.com/NZGX9YH0 (it's huge, that's why i'm using pastebin). I tried "./bowtie version 1.0.1" but it doesn't work : *"Could not locate a Bowtie index corresponding to basename "version"* *Command: ./bowtie version 1.0.1 "* So I tried with this : "./bowtie --version" and get that : *"./bowtie version 1.0.1* *32-bit* *Built on retureau-MacBookPro* *jeudi 5 juin 2014, 10:57:41 (UTC+0200)* *Compiler: gcc version 4.8.1 (Ubuntu 4.8.1-2ubuntu1~12.04) * *Options: -O3 -m32 -Wl,--hash-style=both * *Sizeof {int, long, long long, void*, size_t, off_t}: {4, 4, 8, 4, 4, 8}"* Actually, I installed an older version of "Rbowtie" with install.package (here is the link : http://bioconductor.org/packages/2.12/bioc/html/Rbowtie.html) This one seems to works. Thanks for your help ! Romain. 2014-06-05 8:52 GMT+02:00 Michael Stadler <michael.stadler@fmi.ch>: > Dear Romain, > > Thank you for the report. I am also using Ubuntu 12.04 LTS, and cannot > reproduce your issue, however I am using 64-bit R, while you are using a > 32-bit version. > > Could you test the following things to narrow down the problem: > > 1. what is the output of: uname -a > > 2. can you compile bowtie 1.0.1? > For this, first download bowtie-1.0.1-src.zip from > https://sourceforge.net/projects/bowtie-bio/files/bowtie/1.0.1/ and then > change on the shell to the directory containing the downloaded file and > run the following commands: > unzip bowtie-1.0.1-src.zip > cd bowtie-1.0.1/ > make > Please send me the console output of the 'make'. In my case, I get > something like: > g++ -O3 -m64 ... -DPOPCNT_CAPABILITY ... > and then I find the bowtie binaries: > ./bowtie --version > which produces: > ./bowtie version 1.0.1 > 64-bit > Built on f022c-852634 > Don Jun 5 08:47:09 CEST 2014 > Compiler: gcc version 4.6.3 (Ubuntu/Linaro 4.6.3-1ubuntu5) > Options: -O3 -m64 -Wl,--hash-style=both -DPOPCNT_CAPABILITY > Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} > > 3. if the above does not work, try building again using: > cd .. > rm -rf bowtie-1.0.1 > unzip bowtie-1.0.1-src.zip > cd bowtie-1.0.1/ > POPCNT_CAPABILITY=0 make > and I can test with: > ./bowtie version 1.0.1 > which gives: > 64-bit > Built on f022c-852634 > Don Jun 5 08:50:34 CEST 2014 > Compiler: gcc version 4.6.3 (Ubuntu/Linaro 4.6.3-1ubuntu5) > Options: -O3 -m64 -Wl,--hash-style=both > Sizeof {int, long, long long, void*, size_t, off_t}: {4, 8, 8, 8, 8, 8} > > Let me know if this is not clear. > > Best, > Michael > > > On 04.06.2014 09:20, Romain [guest] wrote: > > Hi, > > > > I'm trying to install "Rbowtie" package, following the instructions here > : http://bioconductor.org/packages/2.12/bioc/html/Rbowtie.html > > > > When I run the command, I got this error message : > > http://pastebin.com/DkEdCV05 > > > > I'm currently running on Ubuntu 12.4 LTS and all others packages seems > to work well. I updated gcc and g++ to the last version aviable. > > May you help me to identify the problem ? > > > > Thanks. > > > > -- output of sessionInfo(): > > > > R version 3.1.0 (2014-04-10) > > Platform: i686-pc-linux-gnu (32-bit) > > > > locale: > > [1] LC_CTYPE=fr_FR.UTF-8 LC_NUMERIC=C > > [3] LC_TIME=fr_FR.UTF-8 LC_COLLATE=fr_FR.UTF-8 > > [5] LC_MONETARY=fr_FR.UTF-8 LC_MESSAGES=fr_FR.UTF-8 > > [7] LC_PAPER=fr_FR.UTF-8 LC_NAME=C > > [9] LC_ADDRESS=C LC_TELEPHONE=C > > [11] LC_MEASUREMENT=fr_FR.UTF-8 LC_IDENTIFICATION=C > > > > attached base packages: > > [1] stats graphics grDevices utils datasets methods base > > > > other attached packages: > > [1] BiocInstaller_1.14.2 > > > > loaded via a namespace (and not attached): > > [1] tools_3.1.0 > > > > > > -- > > Sent via the guest posting facility at bioconductor.org. > > > > -- > -------------------------------------------- > Michael Stadler, PhD > Head of Computational Biology > Friedrich Miescher Institute > Basel (Switzerland) > Phone : +41 61 697 6492 > Fax : +41 61 697 3976 > Mail : michael.stadler@fmi.ch > -------------------------------------------- > > [[alternative HTML version deleted]]
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@michael-stadler-5887
Last seen 2.3 years ago
Switzerland
Hi Romain, Please find my replies below: On 05.06.2014 11:05, Romain Retureau wrote: > Hi, > > here is the output of uname -a : > > /"Linux retureau-MacBookPro 3.2.0-63-generic-pae #95-Ubuntu SMP Thu May > 15 23:26:11 UTC 2014 i686 i686 i386 GNU/Linux//"/ > / > / > I didn't specify it, but i'm running ubuntu in dual bot on a mac book pro. > Fine. > Here is the output of "make" inside the right folder : > http://pastebin.com/NZGX9YH0 (it's huge, that's why i'm using pastebin). > So I tried with this : "./bowtie --version" and get that : > > /"./bowtie version 1.0.1/ > /32-bit/ > /Built on retureau-MacBookPro/ > /jeudi 5 juin 2014, 10:57:41 (UTC+0200)/ > /Compiler: gcc version 4.8.1 (Ubuntu 4.8.1-2ubuntu1~12.04) / > /Options: -O3 -m32 -Wl,--hash-style=both / > /Sizeof {int, long, long long, void*, size_t, off_t}: {4, 4, 8, 4, 4, 8}"/ Here I am confused. The 'make' output that you pasted shows that bowtie is also failing to build, with the same error message as Rbowtie. So it not possible to run './bowtie --version', because there is no bowtie binary... In the pasted output, I can find "... -DPOPCNT_CAPABILITY ...", while the version below was compiled without that. Am I guessing correctly that you compiled with: POPCNT_CAPABILITY=0 make Please let me know exactly what you do and where the output comes from, otherwise it is difficult for me to help. Michael
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@michael-stadler-5887
Last seen 2.3 years ago
Switzerland
Hi Romain, Great, thank you very much for testing. I am copying the list again so others who might run into the problem are aware that an update to Rbowtie version 1.4.5 (release) or 1.5.5 (development) should solve the issue. Best wishes, Michael On 05.06.2014 18:06, Romain Retureau wrote: > Hey, > > I installed the attached version of Rbowtie and it works ! No error, no > warnings and works on R. > > Thanks you very much for the fast help ! > Romain. > > > 2014-06-05 16:01 GMT+02:00 Michael Stadler <michael.stadler at="" fmi.ch=""> <mailto:michael.stadler at="" fmi.ch="">>: > > Hi Romain, > > I think we are getting there. I am replying off-list so I can send you > an attachement with a modified version of Rbowtie for you to test. > > It includes many modifications by the bowtie authors that should fix > your problem, however, I have not yet included the POPCNT_CAPABILITY=0. > In case this does not do it yet, I will add the POPCNT_CAPABILITY=0 and > send you one more version. > > You can install the attached version of Rbowtie from the command > line using: > R CMD INSTALL Rbowtie_1.5.5.tar.gz > > Thanks again for reporting and for testing the modified versions! > Best, > Michael > > > > On 05.06.2014 14:30, Romain Retureau wrote: > > Yes, i'm sorry. The "make" didn't works, but the "POPCNT_CAPABILITY=0 > > make" works. > > > > After installing it by this command "POPCNT_CAPABILITY=0 make" I did > > this "./bowtie --version" and got the message I pasted. > > > > Sorry, it wasn't clear at all. > > Thanks. > > > > > > 2014-06-05 14:08 GMT+02:00 Michael Stadler <michael.stadler at="" fmi.ch=""> <mailto:michael.stadler at="" fmi.ch=""> > > <mailto:michael.stadler at="" fmi.ch="" <mailto:michael.stadler="" at="" fmi.ch="">>>: > > > > Hi Romain, > > > > Please find my replies below: > > > > On 05.06.2014 11:05, Romain Retureau wrote: > > > Hi, > > > > > > here is the output of uname -a : > > > > > > /"Linux retureau-MacBookPro 3.2.0-63-generic-pae #95-Ubuntu SMP > > Thu May > > > 15 23:26:11 UTC 2014 i686 i686 i386 GNU/Linux//"/ > > > / > > > / > > > I didn't specify it, but i'm running ubuntu in dual bot on a mac > > book pro. > > > > > Fine. > > > > > Here is the output of "make" inside the right folder : > > > http://pastebin.com/NZGX9YH0 (it's huge, that's why i'm using > > pastebin). > > > > > So I tried with this : "./bowtie --version" and get that : > > > > > > /"./bowtie version 1.0.1/ > > > /32-bit/ > > > /Built on retureau-MacBookPro/ > > > /jeudi 5 juin 2014, 10:57:41 (UTC+0200)/ > > > /Compiler: gcc version 4.8.1 (Ubuntu 4.8.1-2ubuntu1~12.04) / > > > /Options: -O3 -m32 -Wl,--hash-style=both / > > > /Sizeof {int, long, long long, void*, size_t, off_t}: {4, 4, > 8, 4, > > 4, 8}"/ > > > > Here I am confused. The 'make' output that you pasted shows > that bowtie > > is also failing to build, with the same error message as Rbowtie. > > > > So it not possible to run './bowtie --version', because there > is no > > bowtie binary... > > > > In the pasted output, I can find "... -DPOPCNT_CAPABILITY > ...", while > > the version below was compiled without that. Am I guessing > correctly > > that you compiled with: > > POPCNT_CAPABILITY=0 make > > > > Please let me know exactly what you do and where the output > comes from, > > otherwise it is difficult for me to help. > > > > Michael > > > > > > -- > -------------------------------------------- > Michael Stadler, PhD > Head of Computational Biology > Friedrich Miescher Institute > Basel (Switzerland) > Phone : +41 61 697 6492 <tel:%2b41%2061%20697%206492> > Fax : +41 61 697 3976 <tel:%2b41%2061%20697%203976> > Mail : michael.stadler at fmi.ch <mailto:michael.stadler at="" fmi.ch=""> > -------------------------------------------- > >
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