I am facing a problem of using flowpeaks for a flowframe to get the required clusters.
fp2<-flowPeaks(barcode[,c(1,3)]) how can I replace barcode with a flowframe? can I know the format for R code?
and fpc<-assign.flowPeaks(fp2,fp2$x) what is fp2$x here mean?
May I know
fp.z<-flowPeaks(asinh(samp@exprs[,3:4])) let us say the 2nd column is SSC-A and 4th is FITC-A how can I plot between FITC-A ans SSC-A?