Depth or # of reads for RNASeqPower
0
0
Entering edit mode
drnknmstrr • 0
@drnknmstrr-6906
Last seen 6.7 years ago

Hi,

I am confused by this sentence in the vignette for RNASeqPower:

"The depth of sequencing and consequent expected count µ for a given transcript, argument
depth."

I don't understand how Mu and depth can be used interchangeably. Should this just be the number of reads mapping to a transcript or does this value need to be normalized by transcript length and read length?

cheers,

Ruben

 

 

rnaseqpower • 697 views
ADD COMMENT
0
Entering edit mode

From looking at the code, I believe depth = mu = number of reads.

https://hedgehog.fhcrc.org/bioconductor/branches/RELEASE_3_0/madman/Rpacks/RNASeqPower/R/rnapower.R

ADD REPLY

Login before adding your answer.

Traffic: 316 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6