**10**wrote:

Dear all,

I have a general question regarding RNA-Seq analysis to identify Differentially Expressed Genes (DEGs).

Basically I want to do statistical analysis from one replicate.

I have the following design:

- Group A (time point 1) 2 replicates
- Group B (time point 2) 2 replicates
- Group C (time point 3) 2 replicates
- Group D (time point 4) 1 replicate

My question is whether it is possible for me to infer the dispersion (variance or SD) from Group A, B or C per gene to Group D, to simulate 2 replicates? Dispersions in the group are independent to the time factor.

If so, how should I practically do it?

Thankyou in advance.

Rieza

**38k**• written 4.8 years ago by r.aprianto •

**10**

You say you want to do a "statistical analysis from one replicate". However, you need at least two groups to do a differential expression analysis. Can you clarify your aim here?

24kSorry if the question was unclear.

I have four groups as mentioned above and I want to use the 4th group which only contains 1 replicate as opposed to 2 replicates for the other groups. Differential expression is then performed between those groups.

10