I have a general question regarding RNA-Seq analysis to identify Differentially Expressed Genes (DEGs).
Basically I want to do statistical analysis from one replicate.
I have the following design:
- Group A (time point 1) 2 replicates
- Group B (time point 2) 2 replicates
- Group C (time point 3) 2 replicates
- Group D (time point 4) 1 replicate
My question is whether it is possible for me to infer the dispersion (variance or SD) from Group A, B or C per gene to Group D, to simulate 2 replicates? Dispersions in the group are independent to the time factor.
If so, how should I practically do it?
Thankyou in advance.