Hi everyone,
I have problem in reproducing vignette example from MeSHDbi package.
That is, when I run:
> example("makeGeneMeSHPackage")
It should use preformatted data frames "PAO1" and "metaPAO1" to construct custom org.MeSH.XXX.db package that can subsequently be loaded via library() and queried with Annotation.dbi or RSQLite, or at least that is how other org.MeSH.XXX.db packages behave (org.MeSH.Hsa.db, for instance).
but
> example("makeGeneMeSHPackage") mGMSHP> ## makeGeneMeSHPackage enable users to construct mGMSHP> ## user's own custom MeSH package mGMSHP> mGMSHP> ## this is test data which means the relationship between mGMSHP> ## Entrez gene IDs of Pseudomonas aeruginosa PAO1 mGMSHP> ## and its MeSH IDs. mGMSHP> data(PAO1) mGMSHP> head(PAO1) GENEID MESHID MESHCATEGORY SOURCEID 1 877657 D000265 D 937805 2 877657 D000265 D 937805 3 877657 D001412 B 937805 4 877657 D001412 B 937805 5 877657 D001426 D 937805 6 877657 D001483 G 937805 SOURCEDB 1 Bacillus subtilis subsp. spizizenii str. 168 2 Bacillus subtilis subsp. spizizenii str. 168 3 Bacillus subtilis subsp. spizizenii str. 168 4 Bacillus subtilis subsp. spizizenii str. 168 5 Bacillus subtilis subsp. spizizenii str. 168 6 Bacillus subtilis subsp. spizizenii str. 168 mGMSHP> # We are also needed to prepare meta data as follows. mGMSHP> data(metaPAO1) mGMSHP> metaPAO1 NAME 1 SOURCEDATE 2 SOURCENAME 3 SOURCEURL 4 DBSCHEMA 5 DBSCHEMAVERSION 6 ORGANISM 7 SPECIES 8 package 9 Db type VALUE 1 31-July-2013 2 BLASTP 3 ftp://ftp.ncbi.nlm.nih.gov/gene/DATA/ 4 org.Pae.PAO1.MeSH.db 5 1.0 6 Pseudomonas aeruginosa PAO1 7 8 AnnotationDbi 9 BLASTDb mGMSHP> ## sets up a temporary directory for this example mGMSHP> ## (users won't need to do this step) mGMSHP> destination <- tempfile() mGMSHP> dir.create(destination) mGMSHP> ## makes an Organism package for human called Homo.sapiens mGMSHP> makeGeneMeSHPackage(pkgname = "org.MeSH.Pae.db", mGMSHP+ data = PAO1, mGMSHP+ metadata = metaPAO1, mGMSHP+ organism = "Pseudomonas aeruginosa PAO1", mGMSHP+ version = "1.0.0", mGMSHP+ maintainer = "Koki Tsuyuzaki <k.t.the-answer@hotmail.co.jp>", mGMSHP+ author = "Koki Tsuyuzaki", mGMSHP+ destDir = destination, mGMSHP+ license="Artistic-2.0") Creating package in /var/folders/19/6n33ytjd6nd1m9rtrt6plcfm0000gn/T//RtmpFLPUeT/filef4160c94d3/org.MeSH.Pae.db [1] TRUE Warning messages: 1: 'dbBeginTransaction' is deprecated. Use 'dbBegin' instead. See help("Deprecated") 2: 'dbBeginTransaction' is deprecated. Use 'dbBegin' instead. See help("Deprecated")
so when I try to load it, it says:
> library("org.MeSH.Pae.db") Error in library("org.MeSH.Pae.db") : ‘org.MeSH.Pae.db’ is not a valid installed package
could someone give me a clue what went wrong? Should there be another sequence of commands that should "install" this package properly?
I would really appreciate any help with this.
Best,
Svetlana Bojic, MD
session info output is below:
> sessionInfo() R version 3.1.0 (2014-04-10) Platform: x86_64-apple-darwin10.8.0 (64-bit) locale: [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8 attached base packages: [1] grid parallel stats graphics [5] grDevices utils datasets methods [9] base other attached packages: [1] meshr_1.0.4 [2] org.MeSH.Syn.db_1.0.0 [3] org.MeSH.Bsu.168.db_1.0.0 [4] org.MeSH.Atu.K84.db_1.0.0 [5] org.MeSH.Aca.db_1.0.0 [6] org.MeSH.Hsa.db_1.0.0 [7] org.Hs.eg.db_2.14.0 [8] cummeRbund_2.6.1 [9] Gviz_1.8.4 [10] rtracklayer_1.24.2 [11] GenomicRanges_1.16.4 [12] IRanges_1.22.10 [13] fastcluster_1.1.16 [14] reshape2_1.4.1 [15] ggplot2_1.0.0 [16] Category_2.30.0 [17] GO.db_2.14.0 [18] RSQLite_1.0.0 [19] Matrix_1.1-5 [20] fdrtool_1.2.13 [21] MeSH.PCR.db_1.0.0 [22] MeSH.db_1.0.1 [23] MeSH.AOR.db_1.0.0 [24] MeSHDbi_1.0.2 [25] GSEABase_1.26.0 [26] graph_1.42.0 [27] annotate_1.42.1 [28] AnnotationDbi_1.26.1 [29] GenomeInfoDb_1.0.2 [30] Biobase_2.24.0 [31] BiocGenerics_0.10.0 [32] limma_3.20.9 [33] phyloseq_1.8.2 [34] RMySQL_0.10.2 [35] DBI_0.3.1 [36] viz14_0.0.10 loaded via a namespace (and not attached): [1] acepack_1.3-3.3 [2] ade4_1.6-2 [3] ape_3.2 [4] assertthat_0.1 [5] base64enc_0.1-2 [6] BatchJobs_1.5 [7] BBmisc_1.9 [8] BiocParallel_0.6.1 [9] biom_0.3.12 [10] biomaRt_2.20.0 [11] Biostrings_2.32.1 [12] biovizBase_1.12.3 [13] bitops_1.0-6 [14] brew_1.0-6 [15] BSgenome_1.32.0 [16] checkmate_1.5.1 [17] cluster_2.0.1 [18] codetools_0.2-10 [19] colorspace_1.2-5 [20] data.table_1.9.2 [21] DESeq2_1.4.5 [22] dichromat_2.0-0 [23] digest_0.6.8 [24] dplyr_0.4.1 [25] fail_1.2 [26] foreach_1.4.2 [27] foreign_0.8-63 [28] Formula_1.2-0 [29] genefilter_1.46.1 [30] geneplotter_1.42.0 [31] GenomicAlignments_1.0.6 [32] GenomicFeatures_1.16.3 [33] GenomicFiles_1.0.1 [34] ggbio_1.12.10 [35] ggvis_0.4 [36] gridExtra_0.9.1 [37] gtable_0.1.2 [38] Heatplus_2.10.0 [39] Hmisc_3.15-0 [40] htmltools_0.2.6 [41] httpuv_1.3.2 [42] igraph_0.7.1 [43] iterators_1.0.7 [44] lattice_0.20-30 [45] latticeExtra_0.6-26 [46] locfit_1.5-9.1 [47] magrittr_1.5 [48] MASS_7.3-39 [49] matrixStats_0.14.0 [50] mgcv_1.8-5 [51] mime_0.2 [52] multtest_2.20.0 [53] munsell_0.4.2 [54] nlme_3.1-120 [55] nnet_7.3-9 [56] parody_1.22.0 [57] permute_0.8-3 [58] plyr_1.8.1 [59] proto_0.3-10 [60] R6_2.0.1 [61] RBGL_1.40.1 [62] RColorBrewer_1.1-2 [63] Rcpp_0.11.4 [64] RcppArmadillo_0.4.650.1.1 [65] RCurl_1.95-4.5 [66] RJSONIO_1.3-0 [67] rpart_4.1-9 [68] Rsamtools_1.16.1 [69] scales_0.2.4 [70] sendmailR_1.2-1 [71] shiny_0.11.1 [72] SparseM_1.6 [73] splines_3.1.0 [74] stats4_3.1.0 [75] stringr_0.6.2 [76] survival_2.38-1 [77] tcltk_3.1.0 [78] tools_3.1.0 [79] topGO_2.16.0 [80] VariantAnnotation_1.10.5 [81] vegan_2.2-1 [82] XML_3.98-1.1 [83] xtable_1.7-4 [84] XVector_0.4.0 [85] zlibbioc_1.10.0