Problem importing 5C data
0
0
Entering edit mode
gugpuccio ▴ 10
@gugpuccio-7602
Last seen 3.7 years ago
Finland

Dear All

I'm sorry if this question was answered other times, and if it's a stupid one, but i can't understand how to use the search bar in this site. Every time i look for a word it gives out empy search.

The problem:

I'm trying to import a 5C matrix with the HiTC package in R, but i'm unable to do it. This is the line of code i used:

M=importC(con = "./Desktop/ENR-HeLa-R2.csv", xgi.bed = "./Desktop/ENr.hg19.reverses", ygi.bed = "./Desktop/ENr.hg19.forwards")

And the error message is:

Genomic intervals loaded
Error in HTClist(unlist(obj)) : 
  all elements in '...' must be HTCexp objects
In addition: Warning message:
In mclapply(chromPair, function(chr) { :
  all scheduled cores encountered errors in user code

Then i tried to follow the import tutorial on the HiTC manual, trying to export and then re-importing the data:

E14.bin<-binningC(E14$chrXchrX)
exportC(E14.bin, file="E14")
importC(con="E14.mat", xgi.bed ="E14_xgi.bed", ygi.bed ="E14_ygi.bed")

Getting this error:

Genomic intervals loaded
Loading chrX-chrX...
Error in isN0(as(m, "matrix")) : 
  (list) object cannot be coerced to type 'double'

in this case i used "E14.mat" instead of "E14.count" as written in the tutorial, because it was the only output of the export function, together with the .bed files.

I don't understand what i'm doing wrong. I'm sorry if my question looks stupid, but i'm a newbie in R programming.
Thanks a lot in advance.

 
importing HiTC • 1.1k views
ADD COMMENT

Login before adding your answer.

Traffic: 698 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6