Hi, I was doing part of a RNAseq workflow in R 3.1.1 using command line. But know I want to generate some graph so I decided to copy my RData file to open it in my local computer and continue working with R GUI version (also 3.1.1).

When I open the file I got this messsage:

> load("~/Documents/KIRK_LAB/AD_RNA-seq/results/ale.RData")
Warning: namespace ‘S4Vectors’ is not available and has been replaced
by .GlobalEnv when processing object ‘hse’

I was trying to install S4Vectors but I found out that there is not version for R c 3.1.1

> biocLite("S4Vectors")
BioC_mirror: http://bioconductor.org
Using Bioconductor version 2.14 (BiocInstaller 1.14.3), R version 3.1.1.
Installing package(s) 'S4Vectors'
Warning message:
package ‘S4Vectors’ is not available (for R version 3.1.1) 

How can I have this working?

I will appreciate some help

Hi,

It looks like you generated your RData file with BioC 3.0 and that you are now trying to load it in BioC 2.14 (note that these 2 versions of BioC are based on R 3.1). You cannot in general mix BioC versions. In that particular case, the problem is that the S4Vectors package was introduced in BioC 3.0 and is not available for prior BioC versions. So my advice is that you use BioC >= 3.0 to load your RData file, preferably the current BioC release (BioC 3.1, released last month, and based on R 3.2). Older BioC versions are not supported.

Cheers,

H.