Inconsistency in DESeq2
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Chris • 0
@chris-9148
Last seen 8.5 years ago
United Kingdom

I'm playing around with DESeq2 and I see in the manual that the DESeq() function is a wrapper for estimateSizeFactors(), estimateDispersions() and nbinomWaldTest(). However, when I run the three commands separately I get slightly different results. Specifically the log-fold changes differ in the 2nd decimal place.

So, is DESeq() a wrapper for the functions or a different implementation of the functions? I would expect to get identical results regardless of which way I run DESeq()

 

 

deseq2 • 744 views
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@mikelove
Last seen 4 hours ago
United States
DESeq() also performs outlier replacement in some cases as described in ?DESeq
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What's the purpose of that?

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I'll refer you to the DESeq2 paper and/or vignette for more details.
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OK. I'll look it up.

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@mikelove
Last seen 4 hours ago
United States
DESeq() also performs outlier replacement in some cases as described in ?DESeq
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