getSeq{BSgenome} error: Error in NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append)
2
0
Entering edit mode
komal.rathi ▴ 120
@komalrathi-9163
Last seen 14 months ago
United States

Hi everyone,

I am trying to get the reference base for a set of chromosome-position pairs like this:

library(Rsamtools)
library(BSgenome)
library(BSgenome.Hsapiens.UCSC.hg19)
dat <- read.table(text = 'chr start
chr1  114242
chr1  114242
chr2  7485484', header=T,stringsAsFactors=F)
gr1 <- GRanges(dat$chr,IRanges(start=as.numeric(dat$start), end=as.numeric(dat$start)))
genome <-  getBSgenome("hg19")
refbase <- getSeq(Hsapiens,gr1)

I am getting the following error:

Error in NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) : 
  subscript contains NAs or out-of-bounds indices

No idea what could be causing this, any suggestions?

This is my sessionInfo:

R version 3.2.2 (2015-08-14)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.10.5 (Yosemite)

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] parallel  stats4    stats     graphics  grDevices utils     datasets 
[8] methods   base     

other attached packages:
 [1] BSgenome.Hsapiens.UCSC.hg19_1.4.0 BSgenome_1.38.0                  
 [3] rtracklayer_1.30.1                Rsamtools_1.22.0                 
 [5] Biostrings_2.38.1                 XVector_0.10.0                   
 [7] GenomicRanges_1.22.1              GenomeInfoDb_1.6.1               
 [9] IRanges_2.4.2                     S4Vectors_0.8.3                  
[11] BiocGenerics_0.16.1              

loaded via a namespace (and not attached):
 [1] XML_3.98-1.3               GenomicAlignments_1.6.1   
 [3] bitops_1.0-6               futile.options_1.0.0      
 [5] zlibbioc_1.16.0            futile.logger_1.4.1       
 [7] BiocParallel_1.4.0         lambda.r_1.1.7            
 [9] tools_3.2.2                Biobase_2.30.0            
[11] RCurl_1.95-4.7             SummarizedExperiment_1.0.1
bsgenome rsamtools • 3.2k views
ADD COMMENT •
0
Entering edit mode

Hi,

I can't reproduce this:

library(BSgenome.Hsapiens.UCSC.hg19)
gr1 <- GRanges(c("chr1", "chr2", "chr2"),
               IRanges(c(114242, 114242, 7485484), width=1))
gr1
# GRanges object with 3 ranges and 0 metadata columns:
#       seqnames             ranges strand
#          <Rle>          <IRanges>  <Rle>
#   [1]     chr1 [ 114242,  114242]      *
#   [2]     chr2 [ 114242,  114242]      *
#   [3]     chr2 [7485484, 7485484]      *
#   -------
#   seqinfo: 2 sequences from an unspecified genome; no seqlengths

Then:

getSeq(Hsapiens,gr1)
#   A DNAStringSet instance of length 3
#     width seq
# [1]     1 A
# [2]     1 A
# [3]     1 C

H.

> sessionInfo()
R version 3.2.3 (2015-12-10)
Platform: x86_64-pc-linux-gnu (64-bit)
Running under: Ubuntu 14.04.3 LTS

locale:
 [1] LC_CTYPE=en_US.UTF-8       LC_NUMERIC=C              
 [3] LC_TIME=en_US.UTF-8        LC_COLLATE=en_US.UTF-8    
 [5] LC_MONETARY=en_US.UTF-8    LC_MESSAGES=en_US.UTF-8   
 [7] LC_PAPER=en_US.UTF-8       LC_NAME=C                 
 [9] LC_ADDRESS=C               LC_TELEPHONE=C            
[11] LC_MEASUREMENT=en_US.UTF-8 LC_IDENTIFICATION=C       

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets 
[8] methods   base     

other attached packages:
 [1] BSgenome.Hsapiens.UCSC.hg19_1.4.0 BSgenome_1.38.0                  
 [3] rtracklayer_1.30.1                Biostrings_2.38.3                
 [5] XVector_0.10.0                    GenomicRanges_1.22.3             
 [7] GenomeInfoDb_1.6.1                IRanges_2.4.6                    
 [9] S4Vectors_0.8.6                   BiocGenerics_0.16.1              

loaded via a namespace (and not attached):
 [1] XML_3.98-1.3               Rsamtools_1.22.0          
 [3] GenomicAlignments_1.6.2    bitops_1.0-6              
 [5] futile.options_1.0.0       zlibbioc_1.16.0           
 [7] futile.logger_1.4.1        lambda.r_1.1.7            
 [9] BiocParallel_1.4.3         tools_3.2.3               
[11] Biobase_2.30.0             RCurl_1.95-4.7            
[13] SummarizedExperiment_1.0.2
ADD REPLY •
0
Entering edit mode
tangming2005 ▴ 200
@tangming2005-6754
Last seen 9 weeks ago
United States

I had a similar problem, and it looks like there is a bug in IRanges C: IRanges/ShortRead causing crash?

After updating the packages, getSeq function now works, but the following give me a similar error as this post.

library(GenomicFeatures)
hg19.refseq.db <- makeTxDbFromUCSC(genome="hg19", table="refGene")
refseq.genes<- genes(hg19.refseq.db)

>breakpoint

GRanges object with 10 ranges and 1 metadata column:
       seqnames                 ranges strand |     value
          <Rle>              <IRanges>  <Rle> | <logical>
   [1]     chr1 [  9688116,   9688117]      - |      <NA>
   [2]     chr1 [  9881194,   9881195]      + |      <NA>
   [3]     chr1 [ 48608805,  48608825]      - |      <NA>
   [4]     chr1 [191533733, 191533742]      + |      <NA>
   [5]    chr10 [  6325494,   6325511]      - |      <NA>
   [6]    chr10 [  8130950,   8130958]      - |      <NA>
   [7]    chr10 [  8289372,   8289378]      + |      <NA>
   [8]    chr10 [ 34802689,  34802698]      + |      <NA>
   [9]    chr10 [ 55170266,  55170285]      + |      <NA>
  [10]    chr10 [ 61358365,  61358385]      + |      <NA>


hits <- findOverlaps(breakpoint, refseq.genes, select="arbitrary", ignore.strand=TRUE)

> hits
 [1]    NA  9343 14103  2797    NA    NA    NA 18139    NA    NA

> breakpoint[hits]
Error in NSBS(i, x, exact = exact, upperBoundIsStrict = !allow.append) : 
  subscript contains NAs or out-of-bounds indices

> sessionInfo()
R version 3.2.2 (2015-08-14)
Platform: x86_64-apple-darwin13.4.0 (64-bit)
Running under: OS X 10.10.1 (Yosemite)

locale:
[1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

attached base packages:
[1] stats4    parallel  stats     graphics  grDevices utils     datasets  methods   base     

other attached packages:
 [1] bumphunter_1.10.0                 locfit_1.5-9.1                    iterators_1.0.8                  
 [4] foreach_1.4.3                     GenomicFeatures_1.22.7            AnnotationDbi_1.32.3             
 [7] Biobase_2.30.0                    BSgenome.Hsapiens.UCSC.hg19_1.4.0 BSgenome_1.38.0                  
[10] rtracklayer_1.30.1                Biostrings_2.38.3                 XVector_0.10.0                   
[13] ggplot2_2.0.0                     tidyr_0.3.1                       dplyr_0.4.3                      
[16] GenomicInteractions_1.4.1         GenomicRanges_1.22.2              GenomeInfoDb_1.6.1               
[19] IRanges_2.4.6                     S4Vectors_0.8.5                   BiocGenerics_0.16.1              

loaded via a namespace (and not attached):
 [1] Rcpp_0.12.2                biovizBase_1.18.0          lattice_0.20-33           
 [4] Rsamtools_1.22.0           assertthat_0.1             digest_0.6.8              
 [7] R6_2.1.1                   plyr_1.8.3                 chron_2.3-47              
[10] futile.options_1.0.0       acepack_1.3-3.3            RSQLite_1.0.0             
[13] zlibbioc_1.16.0            data.table_1.9.6           rpart_4.1-10              
[16] labeling_0.3               splines_3.2.2              BiocParallel_1.4.3        
[19] stringr_1.0.0              foreign_0.8-66             igraph_1.0.1              
[22] RCurl_1.95-4.7             biomaRt_2.26.1             munsell_0.4.2             
[25] Gviz_1.14.0                pkgmaker_0.22              nnet_7.3-11               
[28] SummarizedExperiment_1.0.2 gridExtra_2.0.0            Hmisc_3.17-1              
[31] codetools_0.2-14           matrixStats_0.50.1         XML_3.98-1.3              
[34] GenomicAlignments_1.6.1    bitops_1.0-6               grid_3.2.2                
[37] xtable_1.8-0               registry_0.3               gtable_0.1.2              
[40] DBI_0.3.1                  magrittr_1.5               scales_0.3.0              
[43] stringi_1.0-1              doRNG_1.6                  latticeExtra_0.6-26       
[46] futile.logger_1.4.1        Formula_1.2-1              lambda.r_1.1.7            
[49] RColorBrewer_1.1-2         tools_3.2.2                dichromat_2.0-0           
[52] rngtools_1.2.4             survival_2.38-3            colorspace_1.2-6          
[55] cluster_2.0.3              VariantAnnotation_1.16.4

 

 
ADD COMMENT •
2
Entering edit mode

This is just as the error says, you cannot subscript a GRanges or really any Vector with NAs. It is a different problem from the original post.

ADD REPLY •
0
Entering edit mode
komal.rathi ▴ 120
@komalrathi-9163
Last seen 14 months ago
United States

I updated GenomicRanges from GenomicRanges_1.22.1 to GenomicRanges_1.22.2 and it worked. Thanks!
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