Hi,

My flowset is recognized as "null" after the normalization process. Attached below are my codes.

Pls kindly help as I am really not sure what really happened and if required I can send the FCS files as well.

Thanks in advance.

Regards,

Wilson

library(flowCore)
library(flowViz)

library(flowStats)
library(flowClust)
library(parallel)

LChattodata <- list.files("F://26042016/Limited/Control")
LChadata <- read.flowSet(LChattodata)
LChadata
LChadatatrans <- transform("SSC-Log_Height" = log10, "FSC-Log_Height" = log10, "FL1-Log_Height" = log10, "FL2-Log_Height" = log10, "FL3-Log_Height" = log10, "FL4-Log_Height" = log10, "FL5-Log_Height" = log10, "FL6-Log_Height" = log10, "FL7-Log_Height" =log10, "FL8-Log_Height" = log10, "FL9-Log_Height" = log10, "FL10-Log_Height" = log10, "FL11-Log_Height" =log10) %on% LChadata

##Normalisation of the plot##

RectGate <- rectangleGate(filterId="Counts", "FSC-Log_Height"=c(0,5), "SSC-Log_Height"=c(1.3, 5)) #set coordinates first

RectGatedata <- Subset(LChadatatrans, RectGate)

NRectGatedata <- gaussNorm(RectGatedata, "FL1-Log_Height")$LChadatatrans

'gaussNorm' should return a list with items of 'flowSet' and 'confidence', the item ('LChadatatrans') you were referring to simply doesn't exist. Please read the doc and example code in `?gaussNorm`