Microarray normalization---RMA and / or centering+scaling?
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@michaericalribo-11492
Last seen 8.3 years ago

I have an expression dataset from Affy arrays that have been pre-processed with RMA. Is it common to perform further normalization after RMA—eg, de-meaning each gene and scaling by standard deviation—or is this unnecessary due to already being normalized? Thanks!

microarray normalization rma • 1.0k views
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@james-w-macdonald-5106
Last seen 10 hours ago
United States

It's unnecessary.

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