**520**wrote:

Dear all,

I would need a bit of help please on SVA package for batch correction :

https://www.bioconductor.org/packages/release/bioc/html/sva.html

Assuming I have a matrix (let's call it DATA) of gene expression having 3 treatments (lets's say, CTRL, TREAT1, TREAT2), after correcting for batch effects in SVA, how could I obtain the matrix of CORRECTED VALUES ?

A piece of R code is :

DATA= as.matrix(gene.expression) ### here is the MATRIX DATA ;)

group = as.factor(rep(c("CTRL", "TREAT1", "TREAT2"), each=3))

mod1 = model.matrix(~group)

mod0 = cbind(mod1[,1])

svseq = svaseq(DATA, mod1, mod0, n.sv=1)

and I would need to obtain the matrix of CORRECTED_DATA ? thanks !