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peter.rubbens
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@peterrubbens-11606
Last seen 7.6 years ago
Using the package flowTrans to a flowFrame, I get the following error:
Error in .Call("biexponential_transform", y, a, b, c, d, f, w, .Machine$double.eps^0.25, :
"biexponential_transform" not available for .Call() for package "flowCore"
Error in p.ab$convergence : $ operator is invalid for atomic vectors.
How do I solve this?
Thanks, this seems to fix the error (in a number of cases). However, sometimes I get the following error:
Is this due to the fcs-files?
Can you share the reproducible example?
devtools::install_github("RGLab/flowTrans", ref = "trunk") library(flowCore) library(flowTrans) path = "Desktop/directory/fcsfiles" filenames = list.files(path) flowData <- read.flowSet(path = path, transformation = FALSE, pattern = ".fcs") flowframe <- flowData[[1]] colnames <- colnames(flowframe) flowframTrans <- flowTrans(flowframe,'mclMultivBiexp',colnames[1:30], n2f=FALSE, parameters.only = FALSE)I can forward (or share) you an example of an fcs-file as well if you want to.
you can post it as attachment to the github issue or email to wjiang2@fhcrc.org
Or can it be due to the fact that in the example above the scatter and fluorescence variables are all optimalized in the same run, whereas in the paper they are treated separately?