I want to do a time course analyzis with many time points of two conditions but no replicates, following the section 9.6.2 of the Limma User’s Guide (https://www.bioconductor.org/packages/3.3/bioc/vignettes/limma/inst/doc/usersguide.pdf). However, this is for microarray data but my data is RNA-Seq.
The guide says that for RNA-Seq:
"The voom transformation is applied to the read counts. This converts the counts to log-counts per million with associated precision weights. After this, the RNA-seq data can be analyzed as if it was microarray data."
But because I don't have replicates I cannot use voom. Is it OK to use the log2(cpm) I obtained from EdgeR instead of those obtained by voom?