Hi Jose, The additional explanatory variable shouldn't change much for the resampling. If your explanatory variable of interest (group) has 2 values, the two sampled groups should contain the same number of samples with each value. The only thing that is different is that you also need to add gender as explanatory variable to your model (with the original values per sample, so you don't assign new values for this one), assuming you are not interested in finding differentially methylated regions in regard to gender. Cheers, Katja On Tue, Jan 3, 2017 at 2:28 AM, jjalmagro [bioc] <noreply@bioconductor.org> wrote: > Activity on a post you are following on support.bioconductor.org > > User jjalmagro <https: support.bioconductor.org="" u="" 12099=""/> wrote Question: > BiSeq. Model beta regression on resampled data. > <https: support.bioconductor.org="" p="" 90725=""/>: > > Hello, > > I am stuck with one of the steps required to calculated DMRs using BiSeq. > I have modeled the methylation under the beta regression using the group > and gender as explanatory variables. However, when I reach the step where > the beta regression has to be calculated for the resampled data, I am not > sure about how it should be resampled when there are more than one > explanatory variable. Do I have to resample just the group variable or both > of them? > > Regards, > Jose > > ------------------------------ > > Post tags: biseq, methylation, dmr analysis > > You may reply via email or visit BiSeq. Model beta regression on resampled data. >