I'm trying to use WGCNA to analyze a large RNA seq data (~60000 genes). Currently, I am using the codes
blockwiseModules(t(nCount), maxBlockSize = nrow(nCount),power = 5, TOMType = "signed", minModuleSize = 30, reassignThreshold = 0.7, mergeCutHeight = 0.25, numericLabels = TRUE, pamRespectsDendro = FALSE, saveTOMs = TRUE, saveTOMFileBase = "RNATOM", verbose = 3)
When running on a workstation with ~130 G memory, I received a failure message "out of memory". I am setting
maxBlockSize = nrow(nCount) to avoid pre-clustering of genes, but this seems to give rise to this issue. What would be a recommended parameter?