genbankr throwing C stack usage error when reading in files >500Kb
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Lauren • 0
@9541a822
Last seen 2.5 years ago
United States

In my hands, the readGenBank function in the genbankr package works very well with smaller GenBank files (<500 KB), but gives me a C stack usage error with any larger files.

In the example below chrI is 442Kb and chrIII is 638Kb. Am I doing something wrong and/or do the larger files need some pre-treatment?

I've tried other files as well - larger files always throw this error. Small files work just fine. I am currently running genbankr version 1.20.0 on R version 4.1.2.

Thanks!


    > library(genbankr)

    > chrI <- readGenBank("/Users/USER/Desktop/chrI.gb")
    Translation product seems to be missing for 1 of 101 CDS annotations. Setting to ''
    No exons read from genbank file. Assuming sections of CDS are full exons
    No transcript features (mRNA) found, using spans of CDSs

    > chrII <- readGenBank("/Users/USER/Desktop/chrII.gb")
    Error: C stack usage  7970208 is too close to the limit


    > sessionInfo()
    R version 4.1.2 (2021-11-01)
    Platform: x86_64-apple-darwin17.0 (64-bit)
    Running under: macOS Big Sur 11.5.2

    Matrix products: default
    LAPACK: /Library/Frameworks/R.framework/Versions/4.1/Resources/lib/libRlapack.dylib

    locale:
    [1] en_US.UTF-8/en_US.UTF-8/en_US.UTF-8/C/en_US.UTF-8/en_US.UTF-8

    attached base packages:
    [1] stats4    parallel  stats     graphics  grDevices utils     datasets  methods   base     

    other attached packages:
     [1] BSgenome_1.60.0             rtracklayer_1.52.1          GenomicAlignments_1.28.0   
     [4] Rsamtools_2.8.0             Biostrings_2.60.2           XVector_0.32.0             
     [7] SummarizedExperiment_1.22.0 MatrixGenerics_1.4.3        matrixStats_0.61.0         
    [10] GenomeInfoDbData_1.2.6      GenomicFeatures_1.44.2      AnnotationDbi_1.54.1       
    [13] Biobase_2.52.0              GenomicRanges_1.44.0        GenomeInfoDb_1.28.4        
    [16] IRanges_2.26.0              S4Vectors_0.30.2            BiocGenerics_0.38.0        
    [19] genbankr_1.20.0
genbankr • 448 views
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