Hi, I have tried to analyze my scRNA-Seq data set by DESeq2 but as I follow the Recommendations for single-cell analysis,
dds <- DESeq(dds, useT=TRUE, minmu=1e-6, test="LRT", fitType = "glmGamPoi")
I have received this error and couldn't fix it.
Error in DESeq(dds, useT = TRUE, minmu = 1e-06, test = "LRT", : likelihood ratio test requires a 'reduced' design, see ?DESeq
I would appreciate it if someone could help me.