Question

DESeq2 on normalized data

0

Entering edit mode

lizaaa • 0

@8aed39be

Last seen 19 months ago

Russia

I've recently started using DESeq2. Unfortunately, I don't have raw counts data but have normalized counts. How can I analyse gene expression for already normalized counts using DESeq2?

DESeq2 • 936 views

ADD COMMENT • link 20 months ago lizaaa • 0

score 0 · Answer 1 · 2022-08-18

0

Entering edit mode

ATpoint ★ 4.0k

@atpoint-13662

Last seen 10 hours ago

Germany

It’s not supported. Use limma-trend with the log2-transformed normalized counts if that is all you have. See limma manual.

ADD COMMENT • link 20 months ago ATpoint ★ 4.0k

0

Entering edit mode

Thank you!

ADD REPLY • link 20 months ago lizaaa • 0