Hi, I am new to the DESeq2, and I wonder if the dataset (either .csv or .txt) prepared from other program can be imported to DESeq2 as a form of DESeqDataSet in R. The example file is as belows. Any input would be appreciated.

GeneID Length Control Treatment log2Ratio q-value Up-Down-Regulation p-value Symbol
Cfor_09914 879 2297200.96 529502.64 -1.94 0.00E+00 Down 0.00E+00 Cfor1
BGI_novel_G002829 1318 76121 945550 3.81 0.00E+00 Up 0.00E+00 NA
BGI_novel_G001907 1167 195994.23 1006695.3 2.54 0.00E+00 Up 0.00E+00 NA
BGI_novel_G001120 760 728876.52 39924.83 -4.01 0.00E+00 Down 0.00E+00 NA

The counts for DESeq2 must be a numeric matrix with integers (raw counts) where rows are genes /features and columns are samples. At least one of the experimental groups must be n=2 at least to have replication. You seem to have only a control and a treatment column, that makes this unreplicated on the one hand, and also on the other hand this does not look like integer counts. So you cannot use DESeq2. Please refer to the vignette for more help.

The point of DESeq2 is to take counts from groups of samples and calculate logchanges and p values. Since you have that, why do you need DESeq?