I have been trying to perform background correct for the Agilent Single Color Array 8X60K using Limma
But when i had tried it, i am getting the following response.
> backgroundCorrect(X, method="subtract", offset=0) Error in backgroundCorrect(X, method = "subtract", offset = 0) : subtractcorrection requires background intensities
However, the .txt file that i have loaded contains the background intensity information.
Please kindly suggest me how i can rectify this error. I intend to take the background corrected data further into Genespring software.
Thanks for your help in advance