RNA seq with edgeR with several factors
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Nathalie ▴ 20
@ab7ad8dd
Last seen 13 months ago
France

Hi, I am new to bioinformatics. I use EdgeR. I analyze a study in humans with 3 factors, each having about 5 levels. Can this pose a problem for modeling for differential analysis? Can explicatives variables be added to the model? Thank you for your help.

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RNAseq factors • 554 views
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I suggest you go through the extensive edgeR manual which covers all that.

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@gordon-smyth
Last seen 5 hours ago
WEHI, Melbourne, Australia

edgeR handles any number of factors and levels. That's no trouble at all from a software point of view but, naturally, the more effects and coefficients you want to estimate, the more data (samples) you need to have.

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Thank you very much for your help. Sincerely yours, Nathalie

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