Help me In deseq2 design

I have factors sex, 2species, 5stages

I want to know differential expressed genes between male and female of one species and And then from this extract I want to compare male of this species to 2nd species male

In short Regress out female effect from each stage and other species male

I designed like

Design = ~ Replicate + sex + stage + species And made ref = female

And plan to use contrast

I think something is wrong Please help how could I do this in one design I able to extract above objective for all stages

Thanks in advance

Please show code and colData, textual descriptions are hard to follow.