ageData (minfi pipeline)
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Saanchi • 0
@c03f5a0b
Last seen 19 months ago
United States

Hi all,

I am trying to follow the pipeline using minfi, limma, miss methyl etc. using "A cross-package Bioconductor workflow for analysing methylation array data [version 3; peer review: 4 approved]" - Jovana Maksimovic et al" and I am stuck on page 32 of the vignette (differential variability) because age.rgSet cannot be extracted no matter what I try. It does extract age.targets but there is definitely an issue with rgset. I tried redownloading the rdata object and yet it won't work. Since I don't have idat files, I don't know what else to do fix this error. Thanks!

Code should be placed in three backticks as shown below


load(paste(newdir,"ageData.RData",sep="/")) 

# include your problematic code here with any corresponding output 

# please also include the results of running the following in an R session 

Error in h(simpleError(msg, call)) : 
  error in evaluating the argument 'x' in selecting a method for function 'nrow': no slot of name "elementMetadata" for this object of class "RGChannelSet"

sessionInfo( )

R version 4.2.2 (2022-10-31)
Platform: aarch64-apple-darwin20 (64-bit)
Running under: macOS Ventura 13.3.1
ageData missMethyl differentialvariability minfi • 810 views
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In the vignette I see : load(file.path(dataDirectory,"ageData.RData")) Where did you find this code ?

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Hello! Have you resolved this issue? I have also encountered the same problem, and it seems that the age.rgSet provided in the data file is problematic. I am unable to load this data, which is preventing me from proceeding with the analysis. I would appreciate any insights or solutions you can provide.

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