Fishpond with unbalanced dataset
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samuelcjx • 0
@cdc1cdaa
Last seen 18 months ago
United States

I have two conditions: negative control and treatment. Each one has 6 replicates. Then, from PCA analysis and heatmap, I found out that one negative control replicate is very different from the rest of the samples and that it is an outlier, so I decided to remove that sample from the dataset for downstream analysis. I proceeded to use fishpond by calling scaleInfReps(), labelKeep(), and swish(). However, swish() returned the following error.

Error in infRepsArray[, cond2, ] : subscript out of bounds 

I suspect that this has to do with me having 5 samples in one condition and 6 samples in the other. Is that right? What should I do here?

EDIT: Fishpond version is 2.6.0

EDIT2: These replicates are biological, rather than technical.

EDIT3: I will include the PCA plot below

PCAplot

rna-seq salmon fishpond swish • 928 views
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After some debugging, I believe that there is a bug in fishpond codebase. I've opened an issue.

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@mikelove
Last seen 9 days ago
United States

Thanks for the report, I will follow up on GH.

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Thanks again for identifying this, I've pushed what I believe to be a fix in 81a2825.

This is also now pushed to Bioc as version 2.6.1 (release) and 2.7.1 (devel), should be available in 24 hours.

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