Hello everyone! I'm fairly new to R, RStudio, bioconductor etc. so please don't laugh at me if this is a stupid/easy question; I've been trying to install DESeq2, and failing after following every tip I found (disabling the HTTPS thing on global environment, re-installing R and Rstudio, checking that my connection is working and that my firewall is deactivated), for days now. The message I get is:

``

Warning: unable to access index for repository https://bioconductor.org/packages/3.14/bioc/src/contrib: cannot open URL 'https://bioconductor.org/packages/3.14/bioc/src/contrib/PACKAGES' Warning: unable to access index for repository https://bioconductor.org/packages/3.14/data/annotation/src/contrib: cannot open URL 'https://bioconductor.org/packages/3.14/data/annotation/src/contrib/PACKAGES'

Warning: unable to access index for repository https://bioconductor.org/packages/3.14/data/experiment/src/contrib: cannot open URL 'https://bioconductor.org/packages/3.14/data/experiment/src/contrib/PACKAGES'

(etcetc, goes on like this for a while)

``` (etcetc)

I get that the main thing is the "can't access index", which I tried to copy and paste on the browser, and it is indeed a problem because it tells me "Unable to connect An error occurred during a connection to mghp.osn.xsede.org. ". Does someone know how to fix this?

I don't know why the repos for previous releases aren't working currently, but unless you need to use old versions, you should always be running the current version of R/Bioconductor, which is R-4.3.0 and Bioconductor 3.17.

Upgrading will fix your issues.