Is it possible to combine count gene expression data coming from one sequencing technology (RNA seq) with intensity gene expression data coming from another sequencing technology (e.g., illuminex)?
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Sep • 0
@06de5a1f
Last seen 14 months ago
Germany

Dear Sir/Madam,

I have a general question: Is it possible to combine count gene expression data coming from one sequencing technology (RNA seq) with intensity gene expression data coming from another sequencing technology (e.g., illuminex), of course for the same set of genes? If this is possbile, would you please explain the methods I can do that?

Thanks a lot for your kind help in advance.

DESeq2 RNASeqData edgeR limma DifferentialExpression • 675 views
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ATpoint ★ 4.5k
@atpoint-13662
Last seen 1 day ago
Germany

The support site is meant to technical help with the packages. General questions like this should be asked at communities such as biostars.org. Generally, such combinations into a single dataset make little sense since data are completely different in terms of scale and batch effects are inevitably present. If you ask elsewhere please include relevant details, such as the analysis goal and what the experimental setup is.

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