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RNASeqData
•
reset
0
votes
3
replies
273
views
Error when trying to do different pairwise comparisons and lfcshrink than what's listed on resultsNames()
RNASeqData
DESeq2
RNASeq
DifferentialExpression
updated 3 days ago by
ATpoint
★ 4.2k • written 3 days ago by
Mark
• 0
0
votes
3
replies
241
views
DE analysis in EdgeR /Can any one please help me.
RNASeqData
DEGraph
RNASeq
edgeR
DegNorm
updated 16 days ago by
Gordon Smyth
51k • written 16 days ago by
Shaozhen
• 0
0
votes
3
replies
331
views
Combining two bulk RNA seq datasets
RNASeqData
DESeq2
Normalization
5 weeks ago
Tanvi
• 0
1
vote
3
replies
332
views
Including infectivity information to RNA-Seq analysis
RNASeqData
DESeq2
RNA-Seq
RNASeq
updated 6 weeks ago by
James W. MacDonald
66k • written 6 weeks ago by
Ben
▴ 50
0
votes
1
reply
198
views
How to identify transcripts/genes with differential kinetics in two genotypes, using time-course RNA seq data?
RNASeqData
DESeq2
DifferentialExpression
updated 7 weeks ago by
James W. MacDonald
66k • written 7 weeks ago by
ZhuYi
• 0
0
votes
0
replies
147
views
ReactomeGSA for RNASeq
ReactomeGSA
BatchEffect
RNASeqData
10 weeks ago
Shaimaa Gamal
• 0
0
votes
1
reply
254
views
NEED FOR NESSASORY HELP
RNASeqData
edgeR
updated 12 weeks ago by
Gordon Smyth
51k • written 12 weeks ago by
z_bahari
• 0
0
votes
1
reply
196
views
Brachypodium distachyon db
EnrichmentBrowser
BrachypodiumDB
RNASeqData
updated 3 months ago by
James W. MacDonald
66k • written 3 months ago by
Leyre
• 0
3
votes
5
replies
808
views
DESEQ Inquiry
DESeq2
rnaseqGene
RNASeqData
limma
RNASeq
updated 4 months ago by
Michael Love
42k • written 5 months ago by
sap275
▴ 10
0
votes
0
replies
362
views
Differential expression analysis between different species
DEG
RNASeqData
DifferentialExpression
SCBN
RNASeq
5 months ago
Abril
• 0
1
vote
2
replies
1.4k
views
How to combine a bulk and pseudobulk dataset?
DESeq2
limma
RNASeq
RNASeqData
written 14 months ago by
informatician
• 0
1
vote
3
replies
481
views
DESeq2 analysis on time-series RNA experiment
RNASeqData
DESeq2
RNASeq
5 months ago
island
• 0
0
votes
2
replies
373
views
ConsensusClusterPlus dosesn't make an output file
ConsensusClusterPlus
RNASeqData
6 months ago
Diana
• 0
0
votes
1
reply
491
views
WGCNA Soft Power
Normalization
DESeq2
Network
RNASeqData
updated 6 months ago by
ATpoint
★ 4.2k • written 6 months ago by
Ömür Koray
• 0
5
votes
6
replies
722
views
Monotonic genes in results of differential gene expression analysis
DifferentialExpression
rnaseqGene
RNASeqData
6 months ago
Shaimaa Gamal
• 0
1
vote
1
reply
482
views
Deseq2 -Model matrix not full rank error: A complex case
RNASeqData
DifferentialExpression
DESeq2
updated 7 months ago by
ATpoint
★ 4.2k • written 7 months ago by
kubracelikbas4
• 0
0
votes
2
replies
479
views
StringDB: Cannot use payload mechanism to colour nodes
RNASeqData
STRINGdb
DifferentialExpression
GraphAndNetwork
Network
7 months ago
Luca
• 0
0
votes
1
reply
534
views
Interaction terms in DESeq2
RNASeqData
RnaSeqSampleSize
DESeq2
updated 7 months ago by
James W. MacDonald
66k • written 7 months ago by
Mimi
• 0
1
vote
2
replies
587
views
RNASeq time course analysis, missing time points for control
RNASeqData
RnaSeqSampleSize
RNASeq
RNASeqPower
7 months ago
drewhaydensteele
• 0
1
vote
1
reply
431
views
Differential gene expression analysis
RNASeqData
RNAseq123
updated 7 months ago by
ATpoint
★ 4.2k • written 8 months ago by
kaurprab
• 0
1
vote
2
replies
857
views
Different results from blockwiseModules and blockwiseConsensusModules individual TOM
RNASeqData
WGCNA
consensusnetworks
8 months ago • updated 7 months ago
Levi
• 0
0
votes
3
replies
909
views
DESeq: Error in DESeqDataSet(se, design = design, ignoreRank) : some values in assay are negative
RNASeqData
DESeq2
8 months ago
Oenone
• 0
3
votes
4
replies
675
views
Is it appropriate to apply linear mixed models to Voom transformed data?
stats
r
RNASeqData
limma
voom
8 months ago
pl23
• 0
0
votes
1
reply
439
views
DESeq2 and determining effects of treatment on a priori/candidate genes from a bulk-RNAseq experiment
RNASeqData
DifferentialExpression
DESeq2
updated 8 months ago by
Michael Love
42k • written 8 months ago by
Pez
▴ 10
2
votes
2
replies
530
views
DESeq2: Comparison of single clinical sample to 4 normals using tumour cohort to infer dispersion of single sample
GeneExpression
DESeq2
RNASeqData
RNASeq
StatisticalMethod
8 months ago
Manthos
• 0
0
votes
1
reply
500
views
DESeq2 interaction between one continuous variable and one categorical variable
RNASeqData
DESeq2
updated 8 months ago by
Wolfgang Huber
★ 13k • written 8 months ago by
meroy
• 0
0
votes
2
replies
531
views
Is my data too noisy for DESeq? Should I model noise as unwanted variation?
RNASeqData
DESeq2
8 months ago
Ben
• 0
0
votes
2
replies
984
views
MA plot for DESEq2 analysis
RNASeqData
DESeq2
8 months ago
RHEA
• 0
2
votes
3
replies
1.1k
views
Two factor design including interaction effect with DESeq2 in R
DESeq2
RNASeqData
deseq2
ExperimentalDesign
updated 9 months ago by
swbarnes2
★ 1.4k • written 9 months ago by
Sam
• 0
1
vote
1
reply
526
views
Multiple comparison using DESeq2
RNASeqData
RNASeq
DESeq2
updated 9 months ago by
James W. MacDonald
66k • written 9 months ago by
fuqichen1
• 0
1
vote
7
replies
1.0k
views
DuplicateCorrelation() block by duplicate sample or by cell line ?
variancePartition
RNASeqData
limma
BulkRNAseq
RNASeq
updated 7 months ago by
Gordon Smyth
51k • written 9 months ago by
Gimena
• 0
0
votes
1
reply
494
views
Comparing RNASeq data from multiple studies
RNASeqData
RnaSeqSampleSize
RNASeqPower
RNASeq
updated 9 months ago by
Basti
▴ 780 • written 10 months ago by
Luke
• 0
2
votes
4
replies
881
views
Deseq2 calculated log2foldchange not consistent with RPM in IP-small RNA-seq
RNASeqData
DifferentialExpression
SmallRNAData
DESeq2
10 months ago
Shihui
• 0
0
votes
4
replies
814
views
filtering out lowly expressed genes by groups
RNASeqData
DESeq2
updated 10 months ago by
James W. MacDonald
66k • written 10 months ago by
sohail.mbio
▴ 10
3
votes
3
replies
1.6k
views
Differential expression analysis with only FPKM matrix available from total newbie in R
FPKM
RNASeqData
DEAnalysis
limma
10 months ago
Ethan Nguyen
• 0
0
votes
1
reply
798
views
Can I transform already normalized rna-seq data with Z-score?
Z-score
RNASeqData
Deseq
DESeq2
updated 10 months ago by
James W. MacDonald
66k • written 10 months ago by
khaserdene99
• 0
0
votes
2
replies
636
views
RSubread DESeq2
RNASeqData
DifferentialExpression
DESeq2
10 months ago
Beyza
• 0
1
vote
1
reply
566
views
bulk RNA-Seq batch correction
RNASeqData
rnaseqcomp
RNASeq
updated 10 months ago by
ATpoint
★ 4.2k • written 10 months ago by
jg0428
• 0
0
votes
0
replies
391
views
How to use erccdashboard with erccmix="Single" design
RNASeqData
erccdashboard
10 months ago
adi.rotem
• 0
0
votes
1
reply
566
views
Is it possible to combine count gene expression data coming from one sequencing technology (RNA seq) with intensity gene expression data coming from …
DESeq2
RNASeqData
edgeR
limma
DifferentialExpression
updated 11 months ago by
ATpoint
★ 4.2k • written 11 months ago by
Sep
• 0
0
votes
1
reply
615
views
Why was the number of DEG different between DESeq results and count by myself using excel?
RNASeqData
DEGseq
updated 11 months ago by
ATpoint
★ 4.2k • written 11 months ago by
sybyun419
• 0
2
votes
3
replies
1.5k
views
bulk RNA-seq analysis using R : batch effect : One question concerning Combat-seq and one question concerning DESeq2 design = batch + condition
BatchEffect
sva
DESeq2
RNASeqData
limma
updated 11 months ago by
James W. MacDonald
66k • written 11 months ago by
D-Bug
▴ 10
0
votes
2
replies
1.3k
views
understanding differences in RSEM, STAR outputs and featureCounts
RNASeqData
Rsubread
mapping
featurecounts
updated 11 months ago by
James W. MacDonald
66k • written 11 months ago by
vitoriastavis
• 0
0
votes
2
replies
1.2k
views
DESeq2 discrepancy in log2FC significance and normalized counts significance
RNASeqData
DESeq2
12 months ago • updated 11 months ago
Marina
• 0
1
vote
2
replies
1.8k
views
IsoformSwitchAnalyzeR: What are the SV values created in design matrix?
salmon
IsoformSwitchAnalyzeR
tximport
RNASeqData
updated 12 months ago by
k.vitting.seerup
▴ 120 • written 12 months ago by
elva.gypsophila
▴ 10
0
votes
6
replies
899
views
Questionable limma results for differential expression on RNA-seq
RNASeqData
DifferentialExpression
limma
updated 12 months ago by
Gordon Smyth
51k • written 12 months ago by
gld-bioinf
• 0
1
vote
2
replies
625
views
I changed the reference in DGE contrasts by DESeq2, but the results changed just a bit and not in the sign of Log2FC (positive to negative and invers…
RNASeqData
DESeq2
updated 12 months ago by
Michael Love
42k • written 12 months ago by
mohammad.hossein.banabazi
• 0
0
votes
0
replies
412
views
BubbleGUM for multi group comparison of gene set enrichment
RNASeqData
GeneSetEnrichment
multiGSEA
12 months ago
sonal
• 0
1
vote
9
replies
1.0k
views
DESeq2 not replicable results
RNASeqData
DESeq2
updated 12 months ago by
Michael Love
42k • written 12 months ago by
Matteo
• 0
0
votes
0
replies
637
views
News:
Course - Network Analysis in Systems Biology with R/Bioconductor
Bioconductor
Network
RNASeqData
SystemsBiology
12 months ago
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Comment: 'path' must be string
by
James W. MacDonald
66k
Yes. We already knew that from the error message, and I told you that in my previous email. The point wasn't to figure out *where* the err…
Comment: 'path' must be string
by
cpierce2
• 0
I ran the debugger and this is the line that causes the error: ``` if (any(startsWith(pkcData[["RTS_ID"]][1L], "RTS")) & any(startsWith(pr…
Comment: Generating surrogate variables across multiple variables of interest
by
Robert
• 0
Yes, we have several biomarkers, and a proteomic set, so we are characterizing each biomarker by it's proteomic association(s). The use for…
Comment: Error running tximeta
by
Guido Hooiveld
★ 4.0k
FYI (since I just finalized a run with `salmon`). For each sample (`.`) an output folder is created that contains 3 files (`cmd_info.json`…
Comment: Error running tximeta
by
Michael Love
42k
Tximeta uses the full directory. There are files that give the key information about the transcriptome. It's not a good idea to selectively…
Votes
Question: Deconvolution Methods on RNA-Seq Data (Mixed cell types)
Answer: Understanding how to contrast two treatments in DESeq2
Answer: Understanding how to contrast two treatments in DESeq2
Cannot extract TxFeatures for a defined GRanges object
Limma time series spline help
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to
Lluís Revilla Sancho
▴ 730
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to
maltethodberg
▴ 180
Scholar
to
Michael Love
42k
Popular Question
to
Leonardo Collado Torres
★ 1.1k
Scholar
to
Gordon Smyth
51k
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