Help with orgdb for non-model species via makeOrgPackageFromNCBI
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mat149 ▴ 70
@mat149-11450
Last seen 24 days ago
United States

I do not know how to install the end product. I also do not know how to interpret the warning message. Hoping for some insight as to where to go from here. Thanks

    library(AnnotationForge) 

    # https://www.ncbi.nlm.nih.gov/datasets/genome/?taxon=32201\

    org<-makeOrgPackageFromNCBI(version = "0.1",author ="MT",maintainer = "netname@aol.com",outputDir = "C:\\Users\\mat14\\OneDrive\\Desktop\\wd\\C-ill-Pawnee\\db",tax_id = 32201,genus ="Carya",species ="illinoinensis")

If files are not cached locally this may take awhile to assemble a 33 GB cache databse in the NCBIFilesDir directory. Subsequent calls to this function should be faster (seconds). The cache will try to rebuild once per day.Please also see AnnotationHub for some pre-builtOrgDb downloads
        preparing data from NCBI ...
        starting download for 
        [1] gene2pubmed.gz
        [2] gene2accession.gz
        [3] gene2refseq.gz
        [4] gene_info.gz
        [5] gene2go.gz
        getting data for gene2pubmed.gz
        rebuilding the cache
        extracting data for our organism from : gene2pubmed
        getting data for gene2accession.gz
        rebuilding the cache
        extracting data for our organism from : gene2accession
        getting data for gene2refseq.gz
        rebuilding the cache
        extracting data for our organism from : gene2refseq
        getting data for gene_info.gz
        rebuilding the cache
        extracting data for our organism from : gene_info
        getting data for gene2go.gz
        rebuilding the cache
        extracting data for our organism from : gene2go
        processing gene2pubmed
        processing gene_info: chromosomes
        processing gene_info: description
        processing alias data
        processing refseq data
        processing accession data
        processing GO data
        making the OrgDb package ...
        Populating genes table:
        genes table filled
        Populating pubmed table:
        pubmed table filled
        Populating chromosomes table:
        chromosomes table filled
        Populating gene_info table:
        gene_info table filled
        Populating entrez_genes table:
        entrez_genes table filled
        Populating alias table:
        alias table filled
        Populating refseq table:
        refseq table filled
        Populating accessions table:
        accessions table filled
        table metadata filled


Creating package in C:\Users\mat14\OneDrive\Desktop\wd\C-ill-Pawnee\db/org.Cillinoinensis.eg.db 

Now deleting temporary database file

complete!

Warning message:

 **In file.remove(dbFileName) :
          cannot remove file 'C:\Users\mat14\OneDrive\Desktop\wd\C-ill-Pawnee\db/org.Cillinoinensis.eg.sqlite', reason 'Permission denied'**
AnnotationForge makeOrgPackageFromNCBI • 759 views
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@james-w-macdonald-5106
Last seen 1 day ago
United States
install.packages("org.Cillinoinensis.eg.db", repos= NULL, type = "source")
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I tried the code you provided, but it is still having issues. Do you know of any potential work-arounds?

...

Creating package in C:\Users\mat14\OneDrive\Desktop\db/org.Cillinoinensis.eg.db 
Now deleting temporary database file
complete!
[1] "org.Cillinoinensis.eg.sqlite"
Warning message:
In file.remove(dbFileName) :
  cannot remove file 'C:\Users\mat14\OneDrive\Desktop\db/org.Cillinoinensis.eg.sqlite', reason 'Permission denied'

install.packages("org.Cillinoinensis.eg.db", repos= NULL, type = "source")

Installing package into 'C:/Users/mat14/AppData/Local/R/win-library/4.3' (as 'lib' is unspecified)

  • installing source package 'org.Cillinoinensis.eg.db' ... ** using staged installation Error : Invalid DESCRIPTION file

Malformed maintainer field.

See section 'The DESCRIPTION file' in the 'Writing R Extensions' manual. ERROR: installing package DESCRIPTION failed for package 'org.Cillinoinensis.eg.db'

  • removing 'C:/Users/mat14/AppData/Local/R/win-library/4.3/org.Cillinoinensis.eg.db' Warning message: In install.packages("org.Cillinoinensis.eg.db", repos = NULL, type = "source") : installation of package 'org.Cillinoinensis.eg.db' had non-zero exit status
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You got this error: Error : Invalid DESCRIPTION file. Malformed maintainer field.

You used: maintainer = "netname@aol.com" (and author ="MT").

AFAIK it should be: maintainer = "Net Name <netname@aol.com>" (and author ="MT <netname@aol.com>").

Thus add your name, and email should be between <>. To be sure, also do this at the author field.

I also struggled with this a bit before...: problem with makeOrgPackageFromNCBI when making an annotation package

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Thanks for pointing it out - this was the solution. Silly mistake on my end, you saved me from walking in (more) circles

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FWIW, it's only the maintainer field that requires the bracketed email. Including in the author field won't cause an error, but is not necessary.

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