Deleted:How to remove the analysis slots added to my scRNA-Seq and scATAC-Seq data objects in R?
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@mohammedtoufiq91-17679
Last seen 3 months ago
United States

Hi,

I have a question about the removing or deleting the analysis slots added to my scRNA-Seq and scATAC-Seq data objects in R perhaps performed via Seurat and Signac. The reason I am deleting this because, previously a member of our lab had performed this analysis (not sure about the parameters used), and I would like to re-perform the analysis using the from both scRNA-Seq and scATAC-Seq data as we are unable to locate the primary data files.

It seems, in Seurat, an object can contain multiple assays, and each assay can have its own data slots like counts, data, and scale.data. If want to keep only the RNA counts and ATAC starting data and remove all other data slots (like scale.data, neighbors, reduction, etc) or other assays, how can I do this by modifying the object as str(my_data) shows a lengthy data object:

Assays(my_data)

[1] "RNA"   "ATAC"  "peaks"

Thank you,

Toufiq

SingleCellWorkflow SingleCellExperiment SingleCellData ATACSeq SingleCell • 656 views
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