Could SGSeq utilizes "ts" tag produced from minimap2 other than "XS" tag?
0
0
Entering edit mode
jk • 0
@5695cc27
Last seen 4 weeks ago
Taiwan

Hi, I obtained BAM files from minimap2 aligner and I wish to conduct the SGSeq pipeline with them. However, the getBamInfo() requires custom tag from the BAM files. The BAM files produced with minimap2 uses "ts" tag instead.

Custom tag 'XS' not found in BAM file:
   ''
Spliced alignments must include a custom tag 'XS'.
Compatible BAM files can be obtained with an alignment
program for RNA-seq data (e.g. GSNAP, HISAT, STAR, TopHat).

The recommended aligner may not suitable for my case. How could I fix this? I appreciate your help. Thank you very much.

XS-tag SplicedAlignment SGSeq minimap2 • 148 views
ADD COMMENT

Login before adding your answer.

Traffic: 467 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6