Identifying DEGs using limma - NanoString GeoMx-NGS RNA Expression Data
2
0
Entering edit mode
@mohammedtoufiq91-17679
Last seen 29 days ago
United States

Hi,

I am working with NanoString GeoMx-NGS RNA Expression Data. The expression data has undergone appropriate QC, filtering, and Q3 -Third Quartile normalization, then by transforming the Q3 normalized data to log2 normalized data values, I plan to identify differentially expressed genes with duplicateCorrelation to estimate the correlation within subjects or repeated measures before applying lmFit. Can I use limma R package to accomplish this?

Thank you,

Toufiq

NanoStringNCTools limma ExpressionData RNASeq edgeR • 229 views
ADD COMMENT
2
Entering edit mode
@james-w-macdonald-5106
Last seen 9 hours ago
United States

Yes, you can use limma.

0
Entering edit mode
@mohammedtoufiq91-17679
Last seen 29 days ago
United States

James W. MacDonald thank you very much.

ADD COMMENT

Login before adding your answer.

Traffic: 389 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6