Hi,

I am working with NanoString GeoMx-NGS RNA Expression Data. The expression data has undergone appropriate QC, filtering, and Q3 -Third Quartile normalization, then by transforming the Q3 normalized data to log2 normalized data values, I plan to identify differentially expressed genes with duplicateCorrelation to estimate the correlation within subjects or repeated measures before applying lmFit. Can I use limma R package to accomplish this?

Thank you,

Toufiq

Yes, you can use limma.

James W. MacDonald thank you very much.