I am doing a Transcriptomics study, where I have 120 samples. I want to know what criteria should I set to filter counts. Below is what I am using now. But I think it maybe I can set the threshold fo the counts to a higher number. Has anyone have any experience in dealing with such big datasets. ```{r} keep <- rowSums(counts(dds)) >= 5 dds <- dds[keep,]

Ser the vignette https://bioconductor.org/packages/release/bioc/vignettes/DESeq2/inst/doc/DESeq2.html

It covers prefiltering (and everything else).