I'm running a crisprseek to get gRNA for nmecas9. I'm expecting 24 nt output and 6 from my PAM sequence. Below is an iteration that works. However, when I modify the gRNA.size to be 24, I get a weight error. When I change the weight commant to rep(1,24), the error changes to a column error. I am stuck.

library(CRISPRseek)
library(BSgenome.Mmusculus.UCSC.mm10)
library(TxDb.Mmusculus.UCSC.mm10.knownGene)
library(org.Mm.eg.db)

res <- offTargetAnalysis(
    inputFilePath = "classified, snooper!",
    format = c("fasta", "fastq", "bed"),
    header = FALSE,
    gRNAoutputName = "p47_mouse_NNNNCC",
    findgRNAs = TRUE,
    exportAllgRNAs = c("all", "fasta", "genbank", "no"),
    findgRNAsWithREcutOnly = FALSE,
    REpatternFile = REpatternFile_default(),
    minREpatternSize = 4,
    overlap.gRNA.positions = c(17, 18),
    findPairedgRNAOnly = FALSE,
    annotatePaired = TRUE,
    paired.orientation = c("PAMout", "PAMin"),
    enable.multicore = FALSE,
    n.cores.max = 6,
    min.gap = 0,
    max.gap = 20,
    gRNA.name.prefix = NULL,
    gRNA.size = 20,
    PAM = "NNNNCC",
    PAM.size = 6,
    PAM.pattern = "NNNNCN",
    BSgenomeName = BSgenome.Mmusculus.UCSC.mm10,
    genomeSeqFile = NULL,
    chromToSearch = "all",
    chromToExclude = chromToExclude_default,
    max.mismatch = 3,
    allowed.mismatch.PAM = 1,
    gRNA.pattern = NULL,
    baseEditing = FALSE,
    targetBase = "C",
    editingWindow = 4:8,
    editingWindow.offtargets = 4:8,
    primeEditing = FALSE,
    PBS.length = 13L,
    RT.template.length = 8:28,
    RT.template.pattern = "D$",
    corrected.seq = NULL,
    targeted.seq.length.change = NULL,
    bp.after.target.end = 15L,
    target.start = NULL,
    target.end = NULL,
    primeEditingPaired.output = "pairedgRNAsForPE.xls",
    min.score = 0,
    topN = 1000,
    topN.OfftargetTotalScore = 10,
    annotateExon = TRUE,
    txdb = TxDb.Mmusculus.UCSC.mm10.knownGene,
    orgAnn = org.Mm.eg.db,
    ignore.strand = TRUE,
    outputDir = "also classified ya snoop!",
    fetchSequence = TRUE,
    upstream = 200,
    downstream = 200,
    weights = weights_default,
    baseBeforegRNA = 4,
    baseAfterPAM = 3,
    featureWeightMatrixFile = featureWeightMatrixFile_default(),
    useScore = TRUE,
    useEfficacyFromInputSeq = FALSE,
    outputUniqueREs = TRUE,
    foldgRNAs = FALSE,
    gRNA.backbone = gRNA.backbone_default,
    temperature = 37,
    overwrite = FALSE,
    scoring.method = c("Hsu-Zhang", "CFDscore"),
    subPAM.activity = subPAM.activity_default,
    subPAM.position = c(22, 23),
    PAM.location = "3prime",
    rule.set = c("Root_RuleSet1_2014", "Root_RuleSet2_2016", "CRISPRscan", "DeepCpf1"),
    chrom_acc = NULL,
    calculategRNAefficacyForOfftargets = TRUE,
    mismatch.activity.file = mismatch.activity.file_default(),
    predIndelFreq = FALSE,
    predictIndelFreq.onTargetOnly = TRUE,
    method.indelFreq = "Lindel",
    baseBeforegRNA.indelFreq = 13,
    baseAfterPAM.indelFreq = 24,
    findOffTargetsWithBulge = FALSE,
    method.findOffTargetsWithBulge = c("CasOFFinder_v3.0.0b3"),
    DNA_bulge = 2,
    RNA_bulge = 2
  )