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Question: RIPseeker peak calling parameters for TruSeq Samll RNA stranded library
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gravatar for ofonov
3 months ago by
ofonov0
Norway/Oslo
ofonov0 wrote:

Hi,

I got somewhat lost in the parameters of RIPseeker. I would like to do peak calling on the data produced with TruSeq Smalll RNA Illumina protocol. It is a stranded library prep protocol -  sequencing reads from read 1 map to the antisense strand and those from read 2 map to the sense strand.

Should I use

reverseComplement = TRUE or FALSE

strandType = "+", "-", or "*"

 

Thank you!

 

ADD COMMENTlink written 3 months ago by ofonov0
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