Right now I'm planning to perform some machine learning analyses on Nanostring data, and would like to experiment coercing the data to linear by DESeq2's VST functions, as VST has been working fine with out RNA-seq data.
However, unlike limma-voom, there're few guidance for using DESeq(2) on Nanostring data. Simon Anders A: Nanostring ncounterdata - DESeq, and I haven't noticed any discussions otherwise.
So, have anyone here experienced using DESeq(2) with Nanostring counts data? And how do you process the data?