i have 10 samples from Geo data set and there was 1067 probe ids its showed (pvalue<0.05) was set after applying limma code (fdr < 0.5) was set , then 294 probeids with genes symbol and with all the other relevant info. was retrieved from 294 113 was upreg and 181 was down reg.
q1>Is it fine to choose fdr<0.5 because fdr<0.05 ,0.01,0.1 shows no result thats why i choose 0.5 is it acceptable or not this is a question.
q2>some probe ids show entrez id and all the other info. but they don’t show gene symbol so should i consider it or not.