Intramodular connectivity RNA-seq WGCNA
1
0
Entering edit mode
@adrianagallego02-13155
Last seen 5.9 years ago

Cordial greeting

Someone could help me with the script to calculate intramodular connectivity for RNA-seq data?

This is the code that I have so far, is this correct?

module = c("indianred4");

# Select module probes
probes = colnames(datExpr)
inModule = (moduleColors==module);
modProbes = probes[inModule]; 
# Recalculate intramodular connectivity (IMConn)
IMConn = softConnectivity(datExpr[, modProbes]);

# Select the corresponding Topological Overlap
length(inModule)
modTOM = TOM[inModule, inModule];
dimnames(modTOM) = list(modProbes, modProbes)

TOM.mat = as.matrix(mod)

Thanks

wgcna • 1.5k views
ADD COMMENT
0
Entering edit mode
@peter-langfelder-4469
Last seen 7 weeks ago
United States

The code should work. RNA-seq, after suitable normalization and transformation, can be analyzed just any other data. You may want to look at the WGCNA FAQ, especially point 4. Then simply follow the WGCNA tutorials or the code in the book (which you seem to be following already).
 

ADD COMMENT
0
Entering edit mode

Thanks Dr. Langfelder, I have one last question, how can I visualize this intramodular connectivity, for example using Cytoscape?

Thanks

ADD REPLY

Login before adding your answer.

Traffic: 330 users visited in the last hour
Help About
FAQ
Access RSS
API
Stats

Use of this site constitutes acceptance of our User Agreement and Privacy Policy.

Powered by the version 2.3.6