Intramodular connectivity RNA-seq WGCNA
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@adrianagallego02-13155
Last seen 6.5 years ago

Cordial greeting

Someone could help me with the script to calculate intramodular connectivity for RNA-seq data?

This is the code that I have so far, is this correct?

module = c("indianred4");

# Select module probes
probes = colnames(datExpr)
inModule = (moduleColors==module);
modProbes = probes[inModule]; 
# Recalculate intramodular connectivity (IMConn)
IMConn = softConnectivity(datExpr[, modProbes]);

# Select the corresponding Topological Overlap
length(inModule)
modTOM = TOM[inModule, inModule];
dimnames(modTOM) = list(modProbes, modProbes)

TOM.mat = as.matrix(mod)

Thanks

wgcna • 1.8k views
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@peter-langfelder-4469
Last seen 8 weeks ago
United States

The code should work. RNA-seq, after suitable normalization and transformation, can be analyzed just any other data. You may want to look at the WGCNA FAQ, especially point 4. Then simply follow the WGCNA tutorials or the code in the book (which you seem to be following already).
 

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Thanks Dr. Langfelder, I have one last question, how can I visualize this intramodular connectivity, for example using Cytoscape?

Thanks

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