Question

CRISPRseek with Doench 2016 scores

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pavitrarc ▴ 20

@pavitrarc-7234

Last seen 3.4 years ago

United States/Seattle/Fred Hutch

Hi,

Does anyone know if there's a way to run CRISPRseek with the gRNA efficacy scores computed using the updated Doench method?

Thanks!

crisprseek • 1.3k views

ADD COMMENT • link updated 7.5 years ago by Julie Zhu ★ 4.3k • written 7.5 years ago by pavitrarc ▴ 20

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Florence,

it has not been implemented in CRISPRseek yet. Do you see big improvement in the efficacy prediction over the original Doench method? Thanks!

Best regards,

Julie

ADD REPLY • link 7.5 years ago Julie Zhu ★ 4.3k

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Thanks for the quick reply, Julie. I haven't compared them both against our data just yet but it would be great to have the option since the authors claim it's an improvement over the previous algorithm.

ADD REPLY • link 7.5 years ago pavitrarc ▴ 20

score 0 · Answer 1 · 2017-06-29

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Julie Zhu ★ 4.3k

@julie-zhu-3596

Last seen 13 months ago

United States

Thanks, Florence! I will try to add it when I get a chance.

Best,

Julie

ADD COMMENT • link 7.5 years ago Julie Zhu ★ 4.3k